Cell Division Cycle 25 Homolog C (S. Pombe) (CDC25C) antibody

Antigen: Cell Division Cycle 25 Homolog C (S. Pombe) (CDC25C)

Synonyms: Cdc25, CDC25C, CDC25, cdc25, xcdc25c

Clonality: Monoclonal (IMD-25)

Host: Please enquire

Reactivity: Human

Application: Western Blotting (WB)

Catalog no.: ABIN343599

Additional Information

Alternative name: CDC25C

Immunogen: Recombinant human Cdc25c. Purification Purified by the goat anti-mouse IgG affinity chromatography.

Format: Affinity-purified

Isotype: IgG1

Clone: IMD-25

Description: Cell division sycle(CDC25) gene product is a protein-tyrosine phosphatase, activates a partially purified p34(cdc2)/cyclin B complex. The cdc25 protein also shares homology with a protein phosphatase with activity against both tyrosine and serine (and thus probably threonine) phosphate residues. CDC25C is expressed predominantly in G2 phase in healer cells. The human gene encodes a protein with a predicted molecular mass of 53,000 daltons whose C-terminal domain shares about 37% sequence identity with the fission yeast cdc25 mitotic inducer. CDC25C gene is mapped to 5q31.

Specificity: Human. No cross reactivity with other proteins.

Application Details

Application Notes: Western blotting: At 1-2 µg/ml with the appropriate system to detect cdc25c in cells and tissues. Other applications have not been tested. Optimal dilutions should be determined by end user.

Buffer: Lyophilized from 1.2% sodium acetate, with 2mg BSA and 0.2mg NaN3 as preservative.

Storage: At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for longer time. Reconstitution: 1.2% sodium acetate or neutral PBS. If 1ml of PBS is used, the antibody concentration will be 100µg/ml.

Research Area: Cell Cycle

Restrictions: For Research Use only