Calponin antibody

Antigen: Calponin

Clonality: Monoclonal (CP-19)

Host: Please enquire

Reactivity: Human, Mouse (Murine), Rat (Rattus), Chicken

Application: Western Blotting (WB), Immunocytochemistry (ICC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Catalog no.: ABIN343606

Additional Information

Immunogen: Turkey gizzard calponin Purification Purified by the goat anti-mouse IgG affinity chromatography.

Format: Affinity-purified

Isotype: IgG2a

Clone: CP-19

Description: Calponin is a basic 34-kD protein first isolated from chicken gizzard and bovine aorta. It is specifically expressed in smooth muscle and binds calmodulin, actin, and tropomyosin. It is able to inhibit the ATPase activity of myosin and is thought to play a role in smooth muscle contraction. Calponin is a smooth muscle specific, actin-, tropomyosin- and calmodulin-binding protein thought to be involved in some way in the regulation or modulation of contraction.

Specificity: Human, mouse, rat, chicken. No cross reactivity with other proteins.

Application Details

Application Notes: Western blotting: At 0.25-0.5 µg/ml with the appropriate system to detect calponin in cells and tissues. Immunohistochemistry (Paraffin-embedded Sections): At 0.5-1 µg/ml to detect calponin in formalin fixed and paraffin embedded tissues. Immunocytochemistry Suitable Other applications have not been tested. Optimal dilutions should be determined by end user.

Buffer: Lyophilized from 1.2% sodium acetate, with 2mg BSA and 0.2mg NaN3 as preservative.

Storage: At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for longer time. Reconstitution: 1.2% sodium acetate or neutral PBS. If 1ml of PBS is used, the antibody concentration will be 100µg/ml.

Research Area: Stem Cells, Cytoskeleton, Microfilaments

Restrictions: For Research Use only