Calmodulin-Stimulated Calcium-ATPase (Ca2+ ATPase) antibody

Antigen: Calmodulin-Stimulated Calcium-ATPase (Ca2+ ATPase)

Synonyms: Ca-ATPase, Ca-p, Ca-P60, CA-P60A, CaP60A, Ca[2+]ATPase, CG3725, DmelCG3725, dSERCA, Kum, l(2)k08308ab, l(2)k09025, SERCA, SERCA2, ATP2A2, SERCA2A

Clonality: Monoclonal (CAP-6)

Host: Please enquire

Reactivity: Human, Mouse (Murine), Rat (Rattus), Rabbit, Chicken, Snake

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Catalog no.: ABIN343611

Additional Information

Alternative name: Ca++ ATPase

Immunogen: Human erythrocyte Ca2+-ATPase. Purification Purified by the goat anti-mouse IgG affinity chromatography.

Format: Affinity-purified

Isotype: IgG2a

Clone: CAP-6

Description: The Ca(2+)-ATPases are a family of plasma membrane pumps encoded by at least 4 genes: ATP2B1 on chromosome 12q21, ATP2B2, ATP2B3 on Xq28, and ATP2B4 on 1q25. The proteins share 84% amino acid sequence identity and 76% nucleic acid sequence homology. The function of calcium-transporting ATPase found in different membranes is to lower cytoplasmic Ca(2+) concentration by pumping Ca(2+) to luminal or extracellular spaces. Mutations in a plasma membrane Ca(2+)-ATPase gene cause deafness in deafwaddler mice.

Specificity: Human, mouse, rat, rabbit. chicken, snake. No cross reactivity with other proteins.

Application Details

Application Notes: Western blotting: At 4 µg/ml with the appropriate system to detect Ca++ ATPase in cells and tissues. Immunohistochemistry (Paraffin-embedded Sections): At 8 µg/ml to detect Ca++ ATPase in formalin fixed and paraffin embedded tissues. Other applications have not been tested. Optimal dilutions should be determined by end user.

Buffer: Lyophilized from 1.2% sodium acetate, with 2mg BSA and 0.2mg NaN3 as preservative.

Storage: At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for longer time. Reconstitution: 1.2% sodium acetate or neutral PBS. If 1ml of PBS is used, the antibody concentration will be 100µg/ml.

Restrictions: For Research Use only