alpha-Fetoprotein (AFP) antibody

Antigen: alpha-Fetoprotein (AFP)

Synonyms: FETA, HPAFP, AFP, MGC128267

Clonality: Monoclonal (AP-1)

Host: Please enquire

Reactivity: Human

Application: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Catalog no.: ABIN343616

Additional Information

Alternative name: alpha-Fetoprotein, AFP

Immunogen: Human a-fetoprotein Purification Purified by the goat anti-mouse IgG affinity chromatography.

Format: Affinity-purified

Isotype: IgG2a

Clone: AP-1

Description: Alpha-fetoprotein (AFP) is a major plasma protein in the fetus, where it is produced by the yolk sac and liver. Direct confirmation of the assignment of the AFP gene to chromosome 4 by in situ hybridization was provided by Harper and Dugaiczyk (1983), who placed the gene in the q11-q22 region, the same region as the albumin gene. Structure and evolution of human alpha-fetoprotein deduced from partial sequence of cloned cDNA.As the major fetal serum protein, Alpha-fetoprotein is not essential for embryonic development but is required for female fertility.

Specificity: Human. No cross reactivity with other proteins.

Application Details

Application Notes: Immunohistochemistry (Paraffin-embedded Sections): At 2-4 µg/ml to detect AFP in formalin fixed and paraffin embedded tissues. Other applications have not been tested. Optimal dilutions should be determined by end user.

Buffer: Lyophilized from 1.2% sodium acetate, with 2mg BSA and 0.2mg NaN3 as preservative.

Storage: At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for longer time. Reconstitution: 1.2% sodium acetate or neutral PBS. If 1ml of PBS is used, the antibody concentration will be 100µg/ml.

Restrictions: For Research Use only