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CD34 (CD34) antibody

Antigen

CD34 (CD34)
Clonality Polyclonal
Host
Alternatives

Rabbit
Reactivity
Alternatives

Mouse (Murine), Human, Rat (Rattus)
Application
Alternatives Western Blotting (WB)
Catalog no. ABIN343619
Quantity 100ul  (0.5mg/ml)  (Variants)
Price 350.00 $   Plus shipping costs $35.00
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anti-CD34 (CD34) antibody

Additional Information
Application Details Images Alternatives Secondary Antibodies Variants back to top

Alternative name CD34
Swiss-Prot P28906
Immunogen This antibody is produced by immunizing rabbits with a synthetic peptide (KLH-coupled) corresponding to near C-terminal residues of CD34.
Format Liquid
Isotype IgG  (Matching secondary antibodies)
Description CD34 is a heavily glycosylated type I transmembrane molecule, that can be phoshorylated by a variety of kinases including Protein kinase C and Tyrosine kinases. CD34 antigen is expressed on small vessel endothelial cells and tumors of epithelial origin. CD34 is possibly an adhesion molecule with a putative role in early hematopoiesis by mediating the attachment of stem cells to the bone marrow extracellular matrix or directly to stromal cells. It could act as a scaffold for the attachment of lineage specific glycans, allowing stem cells to bind to lectins expressed by stromal cells or other marrow components. So CD34 is considered to be ideal marker for identifying and quantifying hematopoietic progenitor stem cells. With interaction with CrkL, CD34 becomes a substrate for PKC and CD34 surface expression is associated with activation of PKC.

Application Details
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Application Notes Dilution: WB: 1:5,000 ICC: 1:250. Positive Control: Mouse Brain Cell. Cellular Localization: Membrane. Molecular Weight: ~90/150kDa + -.
Concentration 0.5mg/ml
Purification Immunogen affinity purified
Buffer 1*TBS (pH7.4), 0.5%BSA, 25%Glycerol. Preservative: 0.05% Sodium Azide.
Storage Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Research Area Hematopoietic Progenitors, Hematopoietic Stem Cells, Cardiogenesis, Lineage Markers, CD Antigens, Surface Receptors of Immune Cells
Restrictions For Research Use only

Alternatives
Application Details Images Secondary Antibodies Variants back to top

Alternatives for antigen "CD34 (CD34)", type "Antibodies"
Hosts Mouse (140), Rabbit (54), Rat (51), Chicken (1)
Reactivities Human (177), Mouse (Murine) (85), Rat (Rattus) (32), Dog (Canine) (27), Cow (Bovine) (15), Pig (Porcine) (15), Rabbit (15), Monkey (6), Primate (5), Primates (3), Rhesus Monkey (2)
Applications Flow Cytometry (FACS) (187), Western Blotting (WB) (85), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (54), Immunofluorescence (IF) (53), Immunoprecipitation (IP) (42), Immunohistochemistry (Frozen Sections) (IHC (fro)) (37), ELISA (28), Immunohistochemistry (IHC) (13), Immunocytochemistry (ICC) (11), Enzyme Immunoassay (EIA) (6), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (4), Functional Studies (Func) (3), Immunoelectron Microscopy (IEM) (2), Dot Blot (Dot) (1)
Conjugates FITC (34), PE (25), Biotin (16), APC (8), RPE (6), Alexa Fluor 488 (3), Alexa Flour 488 (2), Alexa Fluor 350 (2), Alexa Fluor 555 (2), Alexa Fluor 647 (2), Cy3 (2), Cy5 (2), Cy5,PE (2), Cy5.5 (2), Cy7 (2), Cy7,PE (2), Gold (2), HRP (2), PE,Cy3 (2), PE,Cy5 (2), PE,Cy5.5 (2), PE,Cy7 (2), RBITC (2), Alexa Fluor 700 (1), Cy5.5,PE (1), PE,DY647 (1), PE-Dyomics 647 (1), Pacific Blue (1)
Epitopes pTyr330 (1)