»
Antibodies
»
anti-Cytokeratin 18 (KRT18) antibody
Cytokeratin 18 (KRT18) antibody
| Antigen |
|
| Synonyms |
K18, CK18, Krt1-18, CYK18, KRT18, LOC100008885, MGC109143, krt18 |
| Clonality |
Polyclonal |
|
Host
|
|
|
Reactivity
|
Human (142), Rat (Rattus) (56), Mouse (Murine) (55), Dog (Canine) (41), Rabbit (35), Cow (Bovine) (23), Pig (Porcine) (22), Chicken (20), Horse (Equine) (17), Mammalian (10), Hamster (5), None (4), Sheep (Ovine) (3), Bird (Avian) (2), Cat (Feline) (2), Monkey (1)
|
|
Application
|
Western Blotting (WB) (83), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (77), Flow Cytometry (FACS) (67), Immunofluorescence (IF) (67), Immunohistochemistry (IHC) (33), Immunohistochemistry (Frozen Sections) (IHC (fro)) (28), Enzyme Immunoassay (EIA) (22), ELISA (20), Immunoprecipitation (IP) (18), Immunocytochemistry (ICC) (12), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (4), ELISA (Detection) (2), Immunoelectron Microscopy (IEM) (2)
|
| Catalog no. |
ABIN343625 |
| Quantity |
100ul (0.5mg/ml) |
| Price |
350.00 $ Plus shipping costs $35.00
|
| Shipping to |
|
| Availability |
Ships within 7 to 10 Business Days |
|
Alternative name
|
CK18
|
|
Swiss-Prot
|
P05784
|
|
Immunogen
|
This antibody is produced by immunizing rabbits with a synthetic peptide (KLH-coupled) corresponding to a region of C-terminal residues of mouse CK-18.
|
|
Format
|
Liquid
|
|
Isotype
|
IgG (Matching secondary antibodies)
|
|
Description
|
Cytokeratin 18 is an acidic keratin which is found primarily in non squamous epithelia and is present in a majority of adenocarcinomas and ductal carcinomas but not in squamous cell carcinomas. Cytokeratin 18 exists in combination with Cytokeratin 8, a basic keratin. Hepatocellular carcinomas have been reportedly defined by the use of antibodies that recognize only Cytokeratins 8 and 18.
|
|
Application Notes
|
Dilution: WB: 1:25,000-50,000, ICC 1:200. Positive Control: Mouse liver. Cellular Localization: Cytoplasm, perinuclear region. Molecular Weight: 48kDa.
|
|
Concentration
|
0.5mg/ml
|
|
Purification
|
Immunogen affinity purified
|
|
Buffer
|
1*TBS (pH7.4), 0.5%BSA, 25%Glycerol. Preservative: 0.05% Sodium Azide.
|
|
Storage
|
Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
|
|
Restrictions
|
For Research Use only
|
Alternatives for antigen "Cytokeratin 18 (KRT18)", type "Antibodies"
|
Hosts
|
Mouse (89), Rabbit (77), Guinea Pig (1), Sheep (1)
|
|
Reactivities
|
Human (142), Rat (Rattus) (56), Mouse (Murine) (55), Dog (Canine) (41), Rabbit (35), Cow (Bovine) (23), Pig (Porcine) (22), Chicken (20), Horse (Equine) (17), Mammalian (10), Hamster (5), None (4), Sheep (Ovine) (3), Bird (Avian) (2), Cat (Feline) (2), Monkey (1)
|
|
Applications
|
Western Blotting (WB) (83), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (77), Flow Cytometry (FACS) (67), Immunofluorescence (IF) (67), Immunohistochemistry (IHC) (33), Immunohistochemistry (Frozen Sections) (IHC (fro)) (28), Enzyme Immunoassay (EIA) (22), ELISA (20), Immunoprecipitation (IP) (18), Immunocytochemistry (ICC) (12), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (4), ELISA (Detection) (2), Immunoelectron Microscopy (IEM) (2)
|
|
Conjugates
|
FITC (13), Biotin (11), Alexa Fluor 488 (3), HRP (3), Alexa Flour 488 (2), Alexa Fluor 350 (2), Alexa Fluor 555 (2), Alexa Fluor 647 (2), Cy3 (2), Cy5 (2), Cy5.5 (2), Cy7 (2), Gold (2), PE (2), PE,Cy3 (2), PE,Cy5 (2), PE,Cy5.5 (2), PE,Cy7 (2), RBITC (2), Alexa Fluor 700 (1)
|
|
Epitopes
|
pSer33 (7), C-Term (5), Center (1), N-Term (1)
|