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CD8a Molecule (CD8A) (Chain alpha) antibody

Details for Product No. ABIN343703
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Antigen
Synonyms cd8, LOC100136450, BB154331, Ly-2, Ly-35, Ly-B, Lyt-2, zgc:136643, CD8, Leu2, MAL, p32, RHACD8-4
Epitope
Chain alpha
(45), (11), (7), (5), (3), (3), (3), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1)
Reactivity
Mouse (Murine)
(448), (247), (84), (51), (46), (25), (22), (16), (9), (8), (8), (5), (4), (3), (2), (1)
Host
Rat
(538), (216), (142)
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
(138), (87), (69), (35), (26), (16), (12), (8), (7), (6), (6), (5), (5), (5), (4), (4), (4), (4), (4), (4), (4), (4), (3), (3), (3), (3), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application
Immunocytochemistry (ICC), Functional Studies (Func), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunoprecipitation (IP), Flow Cytometry (FACS)
(642), (165), (114), (106), (95), (86), (57), (52), (42), (38), (22), (15), (5), (3), (3), (2), (1), (1), (1), (1), (1), (1), (1)
Pubmed 9 references available
Quantity 0.1 mg
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Catalog No. ABIN343703
217.80 $
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Immunogen Mouse spleen cells
Clone 53-6-7
Isotype IgG2a
Specificity The rat monoclonal antibody 53-6.7 recognizes mouse CD8a (32-34 kDa, alpha chain of the CD8 antigen).
Purification Purified by protein A
Purity > 95 % (by SDS-PAGE)
Sterility 0.2 µm filtered
Alternative Name CD8A
Background The CD8a (CD8 alpha) subunit of CD8 T cell coreceptor is expressed in CD8 alpha/beta heterodimers on majority of MHC I-restricted conventional T cells and thymocytes and in CD8 alpha/alpha homodimers on subsets of memory T cells, intraepithelial lymphocytes, NK cells, macrophages and dendritic cells. Regulation of CD8 beta level on T cell surface seems to be an important mechanism to control their effector function. Assembly of CD8 alpha/beta but not alpha/alpha dimers is connected with formation or localization to the lipid rafts. Recruiting triggered TCR complexes to these membrane microdomains as well as affinity of TCR to MHC I is modulated by CD8, thereby affecting the functional diversity of the TCR signaling.
Application Notes Flow Cytometry: Recommended dilution: 1.5 µg/mL
Immunohistochemistry (frozen sections) Recommended dilution: 1: 1000
Application note: Formaldehyde fixation is not recommended, acetone fixation is preferred.
Functional Application: Isolation and depletion of CD8+ cells, blocking of cytotoxicity, inhibition of CD8+ T cell proliferation.

Working concentrations should be determined by the investigator.
Restrictions For Research Use only
Concentration 1 mg/mL
Buffer Azide free phosphate buffered saline (PBS), approx. pH 7.4.
Preservative Azide free
Handling Advice Do not freeze.
Storage 4 °C
Storage Comment Store at 2-8 °C. Do not use after expiration date stamped on vial label.
General Ledbetter, Herzenberg: "Xenogeneic monoclonal antibodies to mouse lymphoid differentiation antigens." in: Immunological reviews, Vol. 47, pp. 63-90, 1980 (PubMed).

Takahashi, Nakata, Tanaka et al.: "CD4 and CD8 regulate interleukin 2 responses of T cells." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 89, Issue 12, pp. 5557-61, 1992 (PubMed).

Ledbetter, Rouse, Micklem et al.: "T cell subsets defined by expression of Lyt-1,2,3 and Thy-1 antigens. Two-parameter immunofluorescence and cytotoxicity analysis with monoclonal antibodies modifies current views." in: The Journal of experimental medicine, Vol. 152, Issue 2, pp. 280-95, 1980 (PubMed).

Grabbe, Varga, Beissert et al.: "Beta2 integrins are required for skin homing of primed T cells but not for priming naive T cells." in: The Journal of clinical investigation, Vol. 109, Issue 2, pp. 183-92, 2002 (PubMed).

Hata, Sakaguchi, Yoshitomi et al.: "Distinct contribution of IL-6, TNF-alpha, IL-1, and IL-10 to T cell-mediated spontaneous autoimmune arthritis in mice." in: The Journal of clinical investigation, Vol. 114, Issue 4, pp. 582-8, 2004 (PubMed).

Ko, Ko, Chang et al.: "alpha-Galactosylceramide can act as a nasal vaccine adjuvant inducing protective immune responses against viral infection and tumor." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 175, Issue 5, pp. 3309-17, 2005 (PubMed).

Bouwer, Alberti-Segui, Montfort et al.: "Directed antigen delivery as a vaccine strategy for an intracellular bacterial pathogen." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 103, Issue 13, pp. 5102-7, 2006 (PubMed).

Kamimura, Sawa, Sato et al.: "IL-2 in vivo activities and antitumor efficacy enhanced by an anti-IL-2 mAb." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 177, Issue 1, pp. 306-14, 2006 (PubMed).

Mochimaru, Usui, Yaguchi et al.: "Suppression of alkali burn-induced corneal neovascularization by dendritic cell vaccination targeting VEGF receptor 2." in: Investigative ophthalmology & visual science, Vol. 49, Issue 5, pp. 2172-7, 2008 (PubMed).

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