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Details for Product No. ABIN343703

CD8a Molecule (CD8A) antibody

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Antigen
Synonyms CD8, MAL, p32, Leu2, zgc:136643, cd8, LOC100136450
Reactivity
»Alternatives Mouse (Murine)
Host
Clonality (Clone) Monoclonal ()
Conjugate
»Alternatives Un-conjugated
Application
»Alternatives Flow Cytometry (FACS), Immunoprecipitation (IP), Immunohistochemistry (Frozen Sections) (IHC (fro)), Functional Studies (Func), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC)
Pubmed 9 references available
Catalog no. ABIN343703
Quantity 0.1 mg
Price
217.80 $   Plus shipping costs $45.00
Shipping to
Availability Will be delivered in 4 to 5 Business Days
Immunogen Mouse spleen cells
Clone 53-6-7
Isotype IgG2a
Specificity The rat monoclonal antibody 53-6.7 recognizes mouse CD8a (32-34 kDa, alpha chain of theCD8 antigen).
Purification Purified from hybridoma culture supernatant by protein-A affinity chromatography.
Purity > 95% (by SDS-PAGE)
Alternative Name CD8A
Background The CD8a (CD8 alpha) subunit of CD8 T cell coreceptor is expressed in CD8 alpha/betaheterodimers on majority of MHC I-restricted conventional T cells and thymocytes and inCD8 alpha/alpha homodimers on subsets of memory T cells, intraepithelial lymphocytes, NKcells and dendritic cells. Regulation of CD8 beta level on T cell surface seems to be animportant mechanism to control their effector function. Assembly of CD8 alpha/beta but notalpha/alpha dimers is connected with formation or localization to the lipid rafts. Recruitingtriggered TCR complexes to these membrane microdomains as well as affinity of TCR toMHC I is modulated by CD8, thereby affecting the functional diversity of the TCR signaling.
Research Area CD Antigens, Surface Receptors of Immune Cells, Major Histocompatibility Complex (MHC)
Application Notes Flow Cytometry: Recommended dilution: 1. 5 µg/ml. Immunohistochemistry (paraffin sections) - Immunohistochemistry (frozen sections). Application note: Formaldehyde fixation is not recommended, acetone fixation is preferred. ImmunocytochemistryFunctional ApplicationIsolation and depletion of CD8+ cells, blocking of cytotoxicity, inhibition of CD8+ T cellproliferation.
Restrictions For Research Use only
Concentration 1 mg/ml
Buffer Azide free phosphate buffered saline (PBS), approx. pH 7.4, 0.2 µ m filter sterilized.
Preservative Azide free
Storage 4 °C
Ledbetter, Herzenberg: "Xenogeneic monoclonal antibodies to mouse lymphoid differentiation antigens." in: Immunological reviews, Vol. 47, pp. 63-90, 1980 (PubMed).

Takahashi, Nakata, Tanaka et al.: "CD4 and CD8 regulate interleukin 2 responses of T cells." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 89, Issue 12, pp. 5557-61, 1992 (PubMed).

Ledbetter, Rouse, Micklem et al.: "T cell subsets defined by expression of Lyt-1,2,3 and Thy-1 antigens. Two-parameter immunofluorescence and cytotoxicity analysis with monoclonal antibodies modifies current views." in: The Journal of experimental medicine, Vol. 152, Issue 2, pp. 280-95, 1980 (PubMed).

Grabbe, Varga, Beissert et al.: "Beta2 integrins are required for skin homing of primed T cells but not for priming naive T cells." in: The Journal of clinical investigation, Vol. 109, Issue 2, pp. 183-92, 2002 (PubMed).

Hata, Sakaguchi, Yoshitomi et al.: "Distinct contribution of IL-6, TNF-alpha, IL-1, and IL-10 to T cell-mediated spontaneous autoimmune arthritis in mice." in: The Journal of clinical investigation, Vol. 114, Issue 4, pp. 582-8, 2004 (PubMed).

Ko, Ko, Chang et al.: "alpha-Galactosylceramide can act as a nasal vaccine adjuvant inducing protective immune responses against viral infection and tumor." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 175, Issue 5, pp. 3309-17, 2005 (PubMed).

Bouwer, Alberti-Segui, Montfort et al.: "Directed antigen delivery as a vaccine strategy for an intracellular bacterial pathogen." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 103, Issue 13, pp. 5102-7, 2006 (PubMed).

Kamimura, Sawa, Sato et al.: "IL-2 in vivo activities and antitumor efficacy enhanced by an anti-IL-2 mAb." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 177, Issue 1, pp. 306-14, 2006 (PubMed).

Mochimaru, Usui, Yaguchi et al.: "Suppression of alkali burn-induced corneal neovascularization by dendritic cell vaccination targeting VEGF receptor 2." in: Investigative ophthalmology & visual science, Vol. 49, Issue 5, pp. 2172-7, 2008 (PubMed).

Alternatives for antigen "CD8a Molecule (CD8A)", type "Antibodies"
Hosts (713), (291), (151), (1), (1)
Reactivities (540), (334), (99), (76), (57), (33), (33), (27), (18), (10), (10), (9), (6), (5), (1), (1), (1), (1), (1), (1)
Applications (880), (182), (159), (146), (104), (90), (72), (70), (41), (40), (36), (25), (25), (5), (3), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Conjugates (184), (122), (114), (45), (30), (26), (10), (8), (7), (7), (6), (6), (5), (5), (5), (5), (4), (4), (4), (4), (4), (4), (4), (4), (3), (3), (3), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Epitopes (39), (9), (7), (6), (4), (3), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1)
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