CD8a antibody

Antigen: CD8a

Clonality: Monoclonal (53-6-7)

Host: Rat

Reactivity: Mouse (Murine)

Application: Flow Cytometry (FACS), Immunoprecipitation (IP), Immunohistochemistry (Frozen Sections) (IHC (fro)), Functional Studies (Func), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC)

Catalog no.: ABIN343703

Additional Information

Immunogen: Mouse spleen cells

Format: Azide Free

Isotype: IgG2a  (Matching secondary antibodies)

Clone: 53-6-7

Description: The CD8a (CD8 alpha) subunit of CD8 T cell coreceptor is expressed in CD8 alpha/betaheterodimers on majority of MHC I-restricted conventional T cells and thymocytes and inCD8 alpha/alpha homodimers on subsets of memory T cells, intraepithelial lymphocytes, NKcells and dendritic cells. Regulation of CD8 beta level on T cell surface seems to be animportant mechanism to control their effector function. Assembly of CD8 alpha/beta but notalpha/alpha dimers is connected with formation or localization to the lipid rafts. Recruitingtriggered TCR complexes to these membrane microdomains as well as affinity of TCR toMHC I is modulated by CD8, thereby affecting the functional diversity of the TCR signaling.

Specificity: The rat monoclonal antibody 53-6.7 recognizes mouse CD8a (32-34 kDa, alpha chain of theCD8 antigen).

Application Details

Application Notes: Flow Cytometry: Recommended dilution: 1. 5 µg/ml. Immunohistochemistry (paraffin sections) - Immunohistochemistry (frozen sections). Application note: Formaldehyde fixation is not recommended, acetone fixation is preferred. ImmunocytochemistryFunctional ApplicationIsolation and depletion of CD8+ cells, blocking of cytotoxicity, inhibition of CD8+ T cellproliferation.

Concentration: 1 mg/ml

Purity: > 95% (by SDS-PAGE)

Purification: Purified from hybridoma culture supernatant by affinity chromatography.

Buffer: Azide free phosphate buffered saline (PBS), approx. pH 7.4, 0.2 µ m filter sterilized.

Storage: Store at 2-8 o C. Do not use after expiration date stamped on vial label. For long-term storage aliquot and store at -20 o C. Avoid freeze/thaw cycles.

Research Area: CD Antigens, Surface Receptors of Immune Cells, Major Histocompatibility Complex (MHC)

Restrictions: For Research Use only

Publications

Publications: Ledbetter, Herzenberg: "Xenogeneic monoclonal antibodies to mouse lymphoid differentiation antigens." in: Immunological reviews, Vol. 47, pp. 63-90, 1980 (PubMed).

Takahashi, Nakata, Tanaka et al.: "CD4 and CD8 regulate interleukin 2 responses of T cells." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 89, Issue 12, pp. 5557-61, 1992 (PubMed).

Ledbetter, Rouse, Micklem et al.: "T cell subsets defined by expression of Lyt-1,2,3 and Thy-1 antigens. Two-parameter immunofluorescence and cytotoxicity analysis with monoclonal antibodies modifies current views." in: The Journal of experimental medicine, Vol. 152, Issue 2, pp. 280-95, 1980 (PubMed).

Grabbe, Varga, Beissert et al.: "Beta2 integrins are required for skin homing of primed T cells but not for priming naive T cells." in: The Journal of clinical investigation, Vol. 109, Issue 2, pp. 183-92, 2002 (PubMed).

Hata, Sakaguchi, Yoshitomi et al.: "Distinct contribution of IL-6, TNF-alpha, IL-1, and IL-10 to T cell-mediated spontaneous autoimmune arthritis in mice." in: The Journal of clinical investigation, Vol. 114, Issue 4, pp. 582-8, 2004 (PubMed).

Ko, Ko, Chang et al.: "alpha-Galactosylceramide can act as a nasal vaccine adjuvant inducing protective immune responses against viral infection and tumor." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 175, Issue 5, pp. 3309-17, 2005 (PubMed).

Bouwer, Alberti-Segui, Montfort et al.: "Directed antigen delivery as a vaccine strategy for an intracellular bacterial pathogen." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 103, Issue 13, pp. 5102-7, 2006 (PubMed).

Kamimura, Sawa, Sato et al.: "IL-2 in vivo activities and antitumor efficacy enhanced by an anti-IL-2 mAb." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 177, Issue 1, pp. 306-14, 2006 (PubMed).

Mochimaru, Usui, Yaguchi et al.: "Suppression of alkali burn-induced corneal neovascularization by dendritic cell vaccination targeting VEGF receptor 2." in: Investigative ophthalmology & visual science, Vol. 49, Issue 5, pp. 2172-7, 2008 (PubMed).