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Antibodies
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anti-Protein tyrosine Phosphatase, Receptor Type, C (PTPRC) antibody
Protein tyrosine Phosphatase, Receptor Type, C (PTPRC) antibody
| Antigen |
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| Synonyms |
LCA, LY5, B220, CD45, T200, CD45R, GP180, Lca, RT7, L-CA, PTPRC, si:dkeyp-47c5.1 |
| Clonality |
Monoclonal (EM-05) |
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Host
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Reactivity
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Human (248), Mouse (Murine) (185), Rat (Rattus) (60), Dog (Canine) (32), Chicken (27), Cow (Bovine) (18), Sheep (Ovine) (15), Pig (Porcine) (10), Cat (Feline) (6), Bird (Avian) (5), Goat (3), Primate (3), Guinea Pig (2), Rabbit (2), Monkey (1)
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Application
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 Alternatives Flow Cytometry (FACS), Immunoprecipitation (IP), Western Blotting (WB), Immunocytochemistry (ICC)
Flow Cytometry (FACS) (480), Immunohistochemistry (Frozen Sections) (IHC (fro)) (137), Immunoprecipitation (IP) (108), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (83), Western Blotting (WB) (46), Immunohistochemistry (IHC) (24), Immunofluorescence (IF) (19), ELISA (16), Enzyme Immunoassay (EIA) (8), Immunocytochemistry (ICC) (8), Cytotoxicity Test (CyTox) (4), Functional Studies (Func) (2), Radioimmunoassay (RIA) (2), To be determined by user (TBD) (1)
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| Catalog no. |
ABIN343710 |
| Quantity |
0.1 mg (1 mg/ml) |
| Price |
195.00 $ Plus shipping costs $35.00
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| Shipping to |
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| Availability |
Ships within 3 to 5 Business Days |
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Alternative name
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CD45
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Immunogen
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Murine peripheral blood leukocytes
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Format
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Purified
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Isotype
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IgG (Matching secondary antibodies)
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Clone
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EM-05
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Description
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CD45 (LCA, leukocyte common antigen) is a receptor-type protein tyrosine phosphataseubiquitously expressed in all nucleated hematopoietic cells, comprising approximately 10%of all surface proteins in lymphocytes. CD45 glycoprotein is crucial in lymphocytedevelopment and antigen signaling, serving as an important regulator of Src-family kinases.CD45 protein exists as multiple isoforms as a result of alternative splicing, these isoformsdiffer in their extracellular domains, whereas they share identical transmembrane andcytoplasmic domains. These isoforms differ in their ability to translocate into theglycosphingolipid-enriched membrane domains and their expression depends on cell typeand physiological state of the cell. Besides the role in immunoreceptor signaling, CD45 isimportant in promoting cell survival by modulating integrin-mediated signal transductionpathway and is also involved in DNA fragmentation during apoptosis.
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Specificity
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The antibody EM-05 reacts with mouse CD45 antigen (Leukocyte Common Antigen), asingle chain type I transmembrane protein expressed at high level on cells of hematopoieticorigin, except erythrocytes and platelets.
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Application Notes
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Flow Cytometry: Recommended dilution: 2 µg/ml. Positive control: murine peripheral blood leukocytes
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Concentration
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1 mg/ml
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Purity
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> 95% (by SDS-PAGE)
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Purification
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Purified from hybridoma culture supernatant by hydrophobic charge inductionchromatography followed by precipitation methods.
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Buffer
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Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4
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Storage
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Store at 2-8 o C. Do not use after expiration date stamped on vial label. For long-term storage aliquot and store at -20 o C. Avoid freeze/thaw cycles.
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Research Area
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Immunology, Adaptive Immunity, Hematopoietic Progenitors, Glia marker, CD Antigens, Surface Receptors of Immune Cells
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Restrictions
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For Research Use only
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Alternatives for antigen "Protein tyrosine Phosphatase, Receptor Type, C (PTPRC)", type "Antibodies"
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Hosts
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Mouse (331), Rat (206), Rabbit (18)
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Reactivities
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Human (248), Mouse (Murine) (185), Rat (Rattus) (60), Dog (Canine) (32), Chicken (27), Cow (Bovine) (18), Sheep (Ovine) (15), Pig (Porcine) (10), Cat (Feline) (6), Bird (Avian) (5), Goat (3), Primate (3), Guinea Pig (2), Rabbit (2), Monkey (1)
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Applications
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Flow Cytometry (FACS) (480), Immunohistochemistry (Frozen Sections) (IHC (fro)) (137), Immunoprecipitation (IP) (108), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (83), Western Blotting (WB) (46), Immunohistochemistry (IHC) (24), Immunofluorescence (IF) (19), ELISA (16), Enzyme Immunoassay (EIA) (8), Immunocytochemistry (ICC) (8), Cytotoxicity Test (CyTox) (4), Functional Studies (Func) (2), Radioimmunoassay (RIA) (2), To be determined by user (TBD) (1)
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Conjugates
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FITC (86), PE (55), Biotin (54), APC (28), RPE (14), SPRD (8), Cy5 (6), PE,Cy5 (5), Cy5,PE (4), Cy5.5,PE (4), PE,Cy7 (4), Alexa Fluor 488 (3), Cy7,PE (3), PE,Cy5.5 (3), PerCP (3), APC,Cy7 (2), Alexa Fluor 647 (2), Cy5.5 (2), PE,DY647 (2), Pacific Blue (2), RPE,Cy5 (2), Alexa Fluor 350 (1), Alexa Fluor 555 (1), Alexa Fluor 700 (1), PE,DY590 (1), PE,Texas Red (TR) (1), PE-Dyomics 590 (1), PE-Dyomics 647 (1)
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Epitopes
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C-Term (3)
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