AGR3 antibody
| Antigen |
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| Clonality |
Monoclonal (AGR3-2) |
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Host
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Reactivity
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Application
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 Alternatives Western Blotting (WB), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunocytochemistry (ICC)
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| Catalog no. |
ABIN343712 |
| Quantity |
0.1 mg (1 mg/ml) |
| Price |
403.00 $ Plus shipping costs $35.00
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| Shipping to |
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| Availability |
Ships within 3 to 5 Business days |
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Immunogen
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Purified human AGR3 protein
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Format
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Purified
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Isotype
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IgG1 (Matching secondary antibodies)
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Clone
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AGR3-2
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Description
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AGR3 (Anterior Gradient 3) protein, also known as AG3 (hAG-3, HAG3 in human), orBCMP11, is a secreted cytoplasmic protein which is involved in metastasis induction andp53 tumour supressor inhibition. It may serve as molecular marker and potential therapeutictarget for hormone-responsive breast tumours. Its Xenopus homolog is associated withanteroposterior fate determination during early development.
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Specificity
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The antibody AGR3.2 recognizes epitope QYSQALKKV within AGR3 (AG3) protein (19-20kDa), secreted cytoplasmic protein which can serve as a marker of carcinogenesis.
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Application Notes
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Western Blotting - Recommended dilution: 1 µg/ml. Positive control: T47D breast cancer cell line. Negative control: H1299 lung carcinoma cell line. Immunohistochemistry (frozen sections) - Recommended dilution: 1 µg/ml. Positive tissue: breast cancer. Immunocytochemistry - Recommended dilution: 1 µg/ml. Positive control: T47D breast cancer cell line. Negative control: H1299 lung carcinoma cell line
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Concentration
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1 mg/ml
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Purity
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> 95% (by SDS-PAGE)
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Purification
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Purified from ascites by protein-A affinity chromatography.
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Buffer
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The reagent is provided in phosphate buffered saline (PBS) containing 15 mM sodium azideand 0.2% (w/v) high-grade protease free Bovine Serum Albumin (BSA) as a stabilizing agent.
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Storage
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Store at 2-8 o C. Do not use after expiration date stamped on vial label. For long-term storage aliquot and store at -20 o C. Avoid freeze/thaw cycles.
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Restrictions
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For Research Use only
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Alternatives for antigen "AGR3", type "Antibodies"
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Hosts
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Mouse (5), Rabbit (2)
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Reactivities
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Human (7), Mouse (Murine) (1)
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Applications
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Western Blotting (WB) (6), Immunohistochemistry (Frozen Sections) (IHC (fro)) (5), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (4), Immunocytochemistry (ICC) (3), Flow Cytometry (FACS) (2), Immunohistochemistry (IHC) (2), ELISA (1), ELISA (Detection) (1), Immunofluorescence (IF) (1)
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