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Antibody
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anti-Protein tyrosine Phosphatase Receptor Type C PTPRC APC
Protein tyrosine Phosphatase, Receptor Type, C (PTPRC) antibody (APC)
| Antigen |
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| Synonyms |
LCA, LY5, B220, CD45, T200, CD45R, GP180, Lca, RT7, L-CA |
| Clonality |
Monoclonal (EM-05) |
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Host
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Reactivity
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Human (162), Mouse (Murine) (158), Rat (Rattus) (47), Chicken (24), Dog (Canine) (19), Cow (Bovine) (12), Sheep (Ovine) (12), Pig (Porcine) (6), Bird (Avian) (5), Primate (5), Goat (3), Guinea Pig (2), Rabbit (1)
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Conjugate
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FITC (64), Biotin (45), PE (34), APC (24), RPE (14), Cy5 (10), SPRD (7), Cy5.5 (6), Cy7 (3), PerCP (3), Alexa Fluor 488 (2), PE,Cy5 (2), PE/Cy7 (2), Pacific Blue (2), RPE,Cy5 (2), APC,Cy7 (1), APC/Cy7 (1), Alexa Fluor 647 (1), Alexa Fluor 700 (1), PE,Cy5.5 (1), PE,Cy7 (1), PE,DY590 (1), PE,DY647 (1), PE-Dyomics 590 (1), PE-Dyomics 647 (1), PE/Cy5.5 (1)
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Application
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Flow Cytometry (FACS) (376), Immunohistochemistry (Frozen Sections) (IHC (fro)) (102), Immunoprecipitation (IP) (82), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (51), Western Blotting (WB) (28), Immunohistochemistry (IHC) (20), Immunofluorescence (IF) (11), ELISA (7), Enzyme Immunoassay (EIA) (7), Immunocytochemistry (ICC) (7), Cytotoxicity Test (CyTox) (2), Radioimmunoassay (RIA) (2), Functional Studies (Func) (1)
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| Catalog no. |
ABIN343720 |
| Quantity |
0.1 mg (0.1 mg/ml) |
| Price |
377.00 $ Plus shipping costs $35.00
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| Shipping to |
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| Availability |
Ships within 3 to 5 Business days |
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Alternative name
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CD45
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Immunogen
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Murine peripheral blood leukocytes
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Isotype
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IgG
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Clone
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EM-05
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Description
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CD45 (LCA, leukocyte common antigen) is a receptor-type protein tyrosine phosphataseubiquitously expressed in all nucleated hematopoietic cells, comprising approximately 10%of all surface proteins in lymphocytes. CD45 glycoprotein is crucial in lymphocytedevelopment and antigen signaling, serving as an important regulator of Src-family kinases.CD45 protein exists as multiple isoforms as a result of alternative splicing, these isoformsdiffer in their extracellular domains, whereas they share identical transmembrane andcytoplasmic domains. These isoforms differ in their ability to translocate into theglycosphingolipid-enriched membrane domains and their expression depends on cell typeand physiological state of the cell. Besides the role in immunoreceptor signaling, CD45 isimportant in promoting cell survival by modulating integrin-mediated signal transductionpathway and is also involved in DNA fragmentation during apoptosis.
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Specificity
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The antibody EM-05 reacts with mouse CD45 antigen (Leukocyte Common Antigen), asingle chain type I transmembrane protein expressed at high level on cells of hematopoieticorigin, except erythrocytes and platelets.
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Application Notes
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The reagent is designed for Flow Cytometry analysis. Suggested working concentration is 4 µg/ml. Indicated dilution is recommended startingpoint for use of this product. Working concentrations should be determined by the investigator.
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Concentration
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0.1 mg/ml
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Buffer
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The reagent is provided in phosphate buffered saline (PBS) containing 15 mM sodium azideand 0.2% (w/v) high-grade protease free Bovine Serum Albumin (BSA) as a stabilizing agent.
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Storage
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Store in the dark at 2-8 o C. Do not freeze. Avoid prolonged exposure to light. Do not use after expiration date stamped on vial label.Short-term exposure to room temperature should not affect the quality of the reagent.However, if reagent is stored under any conditions other than those specified, the conditions must be verified by the user.
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Research Area
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Immunology, Adaptive Immunity, Hematopoietic Progenitors, Glia marker, CD Antigens, Surface Receptors of Immune Cells
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Restrictions
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For Research Use only
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Alternatives for antigen "Protein tyrosine Phosphatase, Receptor Type, C (PTPRC)", type "Antibodies"
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Hosts
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Mouse (249), Rat (181), Rabbit (7)
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Reactivities
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Human (162), Mouse (Murine) (158), Rat (Rattus) (47), Chicken (24), Dog (Canine) (19), Cow (Bovine) (12), Sheep (Ovine) (12), Pig (Porcine) (6), Bird (Avian) (5), Primate (5), Goat (3), Guinea Pig (2), Rabbit (1)
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Applications
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Flow Cytometry (FACS) (376), Immunohistochemistry (Frozen Sections) (IHC (fro)) (102), Immunoprecipitation (IP) (82), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (51), Western Blotting (WB) (28), Immunohistochemistry (IHC) (20), Immunofluorescence (IF) (11), ELISA (7), Enzyme Immunoassay (EIA) (7), Immunocytochemistry (ICC) (7), Cytotoxicity Test (CyTox) (2), Radioimmunoassay (RIA) (2), Functional Studies (Func) (1)
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Conjugates
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FITC (64), Biotin (45), PE (34), APC (24), RPE (14), Cy5 (10), SPRD (7), Cy5.5 (6), Cy7 (3), PerCP (3), Alexa Fluor 488 (2), PE,Cy5 (2), PE/Cy7 (2), Pacific Blue (2), RPE,Cy5 (2), APC,Cy7 (1), APC/Cy7 (1), Alexa Fluor 647 (1), Alexa Fluor 700 (1), PE,Cy5.5 (1), PE,Cy7 (1), PE,DY590 (1), PE,DY647 (1), PE-Dyomics 590 (1), PE-Dyomics 647 (1), PE/Cy5.5 (1)
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Alternatives 
