CD34 antibody (PE)

Catalog No. ABIN343729

Product Details anti-CD34 Antibody (PE)

Target: CD34

Reactivity: Human, Non-Human Primate

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD34 antibody is conjugated to PE

Application: Flow Cytometry (FACS)

Specificity: The antibody QBEnd-10 reacts with an extracellular class II epitope on CD34, a 110-115 kDa monomeric transmembrane phosphoglycoprotein expressed on hematopoietic progenitors cells and on the most pluripotential stem cells, it is gradually lost on progenitor cells. This antibody has been also used as an endothelial marker.

No Cross-Reactivity: Cow, Dog, Rat, Sheep

Cross-Reactivity (Details): Human, Non-Human Primates

Purification: Purified antibody is conjugated with R-phycoerythrin (PE) under optimum conditions. Unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.

Immunogen: Human endothelial vesicles

Clone: QBEnd-10

Isotype: IgG1

Application Details

Application Notes: Flow cytometry: The reagent is designed for analysis of human blood cells using 20 μL reagent / 100 μL of whole blood or 10⁶ cells in a suspension. The content of a vial (2 ml) is sufficient for 100 tests.

Comment:

The purified antibody is conjugated with R-Phycoerythrin (PE) under optimum conditions. The conjugate is purified by size-exclusion chromatography and adjusted for direct use. No reconstitution is necessary.

Restrictions: For Research Use only

Handling

Reconstitution: No reconstitution is necessary.

Buffer: Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Do not freeze.
Avoid prolonged exposure to light.

Storage: 4 °C

Storage Comment: Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.

References for anti-CD34 antibody (PE) (ABIN343729)

Poblet, Jimenez-Acosta, Rocamora: "QBEND/10 (anti-CD34 antibody) in external root sheath cells and follicular tumors." in: Journal of cutaneous pathology, Vol. 21, Issue 3, pp. 224-8, (1994) (PubMed).

Traoré, Hirn: "Certain anti-CD34 monoclonal antibodies induce homotypic adhesion of leukemic cell lines in a CD18-dependent and a CD18-independent way." in: European journal of immunology, Vol. 24, Issue 10, pp. 2304-11, (1994) (PubMed).

Grimsley, Amos, Gordon, Greaves: "Rapid positive selection of CD34+ cells using magnetic microspheres coated with monoclonal antibody QBEND/10 linked via a cleavable disulphide bond." in: Leukemia, Vol. 7, Issue 6, pp. 898-908, (1993) (PubMed).

Kuzu, Bicknell, Harris, Jones, Gatter, Mason: "Heterogeneity of vascular endothelial cells with relevance to diagnosis of vascular tumours." in: Journal of clinical pathology, Vol. 45, Issue 2, pp. 143-8, (1992) (PubMed).

Sutherland, Marsh, Davidson, Baker, Keating, Mellors: "Differential sensitivity of CD34 epitopes to cleavage by Pasteurella haemolytica glycoprotease: implications for purification of CD34-positive progenitor cells." in: Experimental hematology, Vol. 20, Issue 5, pp. 590-9, (1992) (PubMed).

Ramani, Bradley, Fletcher: "QBEND/10, a new monoclonal antibody to endothelium: assessment of its diagnostic utility in paraffin sections." in: Histopathology, Vol. 17, Issue 3, pp. 237-42, (1991) (PubMed).

Schlingemann, Rietveld, de Waal, Bradley, Skene, Davies, Greaves, Denekamp, Ruiter: "Leukocyte antigen CD34 is expressed by a subset of cultured endothelial cells and on endothelial abluminal microprocesses in the tumor stroma." in: Laboratory investigation; a journal of technical methods and pathology, Vol. 62, Issue 6, pp. 690-6, (1990) (PubMed).

Target Details for CD34

Target: CD34

Alternative Name: CD34 (CD34 Products)

Synonyms: AU040960 antibody, CD34 molecule antibody, CD34 antigen antibody, CD34 antibody, Cd34 antibody

Background: CD34 Molecule,CD34 is a highly glycosylated monomeric 111-115 kDa surface protein, which is present on many stem cell populations. It is a well established stem cell marker, though its expression on human hematopoietic stem cells is reversible. CD34 probably serves as a surface receptor that undergoes receptor-mediated endocytosis and regulates adhesion, differentiation and proliferation of hematopoietic stem cells and other progenitors. CD34 expression is likely to represent a specific state of hematopoietic development that may have altered adhering properties with expanding and differentiating capabilities in both in vitro and in vivo conditions.

Gene ID: 947

UniProt: P28906