Ne (G-L-Glutamyl)-L-Lysine Isopeptide antibody

Antigen: Ne (G-L-Glutamyl)-L-Lysine Isopeptide

Clonality: Monoclonal (Cov81D4)

Host: Mouse

Application: Immunohistochemistry (IHC)

Catalog no.: ABIN346815

Additional Information

Immunogen: Ne (g-L-glutamyl)-L-lysine isopeptide

Format: Ascites

Isotype: IgM kappa  (Matching secondary antibodies)

Clone: Cov81D4

Specificity: Monoclonal antibody reacts with Ne (g-L-glutamyl)-L-lysine. Cross Reactivities: Cross reacts in Elisa with N1,N4 bis (gPoly L-glutamyl) Putrescine and N1,N8 bis (gPoly L-glutamyl) Spermidine. There is no cross reaction with : - protein bound Ne acetyl lysine - protein bound N1 or N4 mono (gPoly L-glutamyl) Putrescine - protein bouprotein bound N1 mono (gPoly L-glutamyl) Spermidine - protein bound Ne (g -L-glutamyl) lysine having a free alpha amine either on its glutamyl or lysyl moieties.

Application Details

Application Notes: Optimal dilutions should be determined by the end user. The following are guidelines only: Immunohistology : 1/50 to 1/800

Buffer: Lyophilized from 200 µl of ascitic fluid. Preservative: None.

Storage: Short term storage: +4°C. Long term storage: -20°C

Restrictions: For Research Use only

Publications

Publications: Roch, Noel, el Alaoui et al.: "Differential expression of isopeptide bonds N epsilon (gamma-glutamyl) lysine in benign and malignant human breast lesions: an immunohistochemical study." in: International journal of cancer. Journal international du cancer, Vol. 48, Issue 2, pp. 215-20, 1991 (PubMed).

el Alaoui, Legastelois, Roch et al.: "Transglutaminase activity and N epsilon (gamma glutamyl) lysine isopeptide levels during cell growth: an enzymic and immunological study." in: International journal of cancer. Journal international du cancer, Vol. 48, Issue 2, pp. 221-6, 1991 (PubMed).

Johnson, LeShoure: "Immunoblot analysis reveals that isopeptide antibodies do not specifically recognize the epsilon-(gamma-glutamyl)lysine bonds formed by transglutaminase activity." in: Journal of neuroscience methods, Vol. 134, Issue 2, pp. 151-8, 2004 (PubMed).

Thomas, Fournet, Simonet et al.: "Definition of the fine specificity of the monoclonal antibody 81D4: its reactivity with lysine and polyamine isopeptide cross-links." in: Journal of immunological methods, Vol. 292, Issue 1-2, pp. 83-95, 2004 (PubMed).

Del Duca, Cai, Di Sandro et al.: "Compatible and self-incompatible pollination in Pyrus communis displays different polyamine levels and transglutaminase activity." in: Amino acids, Vol. 38, Issue 2, pp. 659-67, 2010 (PubMed).

Wang, Wilhelmsson, Hyrsl et al.: "Pathogen entrapment by transglutaminase--a conserved early innate immune mechanism." in: PLoS pathogens, Vol. 6, Issue 2, pp. e1000763, 2010 (PubMed).

Ohtake, Kobayashi, Maruko et al.: "Norepinephrine Modulates the Zonally Different Hepatocyte Proliferation through the Regulation of Transglutaminase Activity." in: American journal of physiology. Gastrointestinal and liver physiology, 2010 (PubMed).