Ganglioside GD3 antibody

Antigen: Ganglioside GD3

Clonality: Monoclonal (Cov4F6)

Host: Mouse

Application: ELISA, Western Blotting (WB), Immunohistochemistry (IHC)

Catalog no.: ABIN346818

Additional Information

Alternative name: Ganglioside-GD3

Immunogen: Ganglioside-GD3

Format: Tissue culture supernatant

Isotype: IgG3 kappa  (Matching secondary antibodies)

Clone: Cov4F6

Specificity: Monoclonal antibody reacts specifically with GD3 ganglioside : (NeuAc alpha 2-8NeuAc alpha 2-3Gal beta 1-4Glc beta 1-1Cer). Cross Reactivities: Cross reacts with 9-O-AcGD3 ganglioside (9OAcNeuAc alpha 2-8NeuAc alpha 2-3Gal beta 1-4Glc beta 1-1Cer) and GT1a ganglioside (NeuAc alpha 2-8NeuAc alpha 2-3Gal beta 1-4GalNAc beta 1-4(NeuAc alpha 2-3)Gal beta 1-4Glc beta 1-1Cer)

Application Details

Application Notes: Optimal dilutions should be determined by the end user. The following are guidelines only: ELISA : 1/5 to 1/50. Western Blot : 1/5 to 1/20

Buffer: Lyophilized from 0,5 ml of tissue culture supernatant. Preservative: None.

Storage: Short term storage: +4°C. Long term storage: -20°C

Restrictions: For Research Use only

Images

Immunological staining of GD3 and melanoma by monoclonal antibody 4F6 (Taki et al., Biochimica et Biophysica Acta 1780 (2008) 497–503)

Publications

Publications: Zebda, Pedron, Rebbaa et al.: "Deficiency of ganglioside biosynthesis in metastatic human melanoma cells: relevance of CMP-NeuAc:LacCer alpha 2-3 sialyltransferase (GM3 synthase)." in: FEBS letters, Vol. 362, Issue 2, pp. 161-4, 1995 (PubMed).

Portoukalian, David, Gain et al.: "Shedding of GD2 ganglioside in patients with retinoblastoma." in: International journal of cancer. Journal international du cancer, Vol. 53, Issue 6, pp. 948-51, 1993 (PubMed).

Thomas, Buronfosse, Combaret et al.: "Gangliosides protect human melanoma cells from ionizing radiation-induced clonogenic cell death." in: Glycoconjugate journal, Vol. 13, Issue 3, pp. 377-84, 1997 (PubMed).

Cerato, Birkle, Portoukalian et al.: "Variable region gene segments of nine monoclonal antibodies specific to disialogangliosides (GD2, GD3) and their O-acetylated derivatives." in: Hybridoma, Vol. 16, Issue 4, pp. 307-16, 1997 (PubMed).

Ha, Lee, Kim: "Overexpression of GD3 synthase induces apoptosis of vascular endothelial ECV304 cells through downregulation of Bcl-2." in: FEBS letters, Vol. 568, Issue 1-3, pp. 183-7, 2004 (PubMed).

Kang, Kang, Kang et al.: "Transcriptional regulation of the human GD3 synthase gene expression in Fas-induced Jurkat T cells: a critical role of transcription factor NF-kappaB in regulated expression." in: Glycobiology, Vol. 16, Issue 5, pp. 375-89, 2006 (PubMed).

Popa, Pons, Mariller et al.: "Purification and structural characterization of de-N-acetylated form of GD3 ganglioside present in human melanoma tumors." in: Glycobiology, Vol. 17, Issue 4, pp. 367-73, 2007 (PubMed).

Taki, Ishikawa, Ogino et al.: "A new approach for drug discovery from glycobiology and phage-displayed peptide library technology." in: Biochimica et biophysica acta, Vol. 1780, Issue 3, pp. 497-503, 2008 (PubMed).

Mitchell, Sullam: "Streptococcus mitis phage-encoded adhesins mediate attachment to {alpha}2-8-linked sialic acid residues on platelet membrane gangliosides." in: Infection and immunity, Vol. 77, Issue 8, pp. 3485-90, 2009 (PubMed).