Ransglutaminase 3 (E Polypeptide, Protein-Glutamine-gamma-Glutamyltransferase) (TGM3) antibody

Antigen: Ransglutaminase 3 (E Polypeptide, Protein-Glutamine-gamma-Glutamyltransferase) (TGM3)

Synonyms: TGE, MGC126249, MGC126250, TGM3, MGC155017

Clonality: Monoclonal (CovB5D)

Host: Mouse

Reactivity: Human

Application: Immunocytochemistry (ICC), Western Blotting (WB)

Catalog no.: ABIN346898

Additional Information

Alternative name: Epidermal Transglutaminase 3 (Tgase 3, clone B5D)

Immunogen: Recombinant human transglutaminase-3

Format: Ascites

Isotype: IgG1 kappa  (Matching secondary antibodies)

Clone: CovB5D

Specificity: Monoclonal antibody reacts specifically with epidermal Transglutaminase 3 (TGase 3) from human epidermal cells and recombinant human transglutaminase 3 (rh-Tgase 3)It recognizes both full length (77 kDa) and proteolysed form (47 kDa). Cross Reactivities: Not yet tested

Application Details

Application Notes: Optimal dilutions should be determined by the end user. The following are guidelines only: Immunocytochemistry : 1/100 to 1/500. Western Blot : 1/200 to 1/1 000

Buffer: Lyophilized from 100 µl of ascitic fluid. Preservative: None.

Storage: Short term storage: +4°C. Long term storage: -20°C

Restrictions: For Research Use only

Publications

Publications: Hitomi, Presland, Nakayama et al.: "Analysis of epidermal-type transglutaminase (transglutaminase 3) in human stratified epithelia and cultured keratinocytes using monoclonal antibodies." in: Journal of dermatological science, Vol. 32, Issue 2, pp. 95-103, 2003 (PubMed).

Hitomi, Ikeda, Maki: "Immunological detection of proteolytically activated epidermal-type transglutaminase (TGase 3) using cleavage-site-specific antibody." in: Bioscience, biotechnology, and biochemistry, Vol. 67, Issue 11, pp. 2492-4, 2003 (PubMed).