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Peptidyl Arginine Deiminase, Type IV (PADI4) (C-Term) antibody

Details for Product No. ABIN347069, Supplier: Log in to see
Antigen
  • PAD
  • PAD4
  • PADI5
  • PDI4
  • PDI5
  • Pad4
  • Pdi4
Epitope
C-Term
15
13
4
4
2
2
2
2
2
2
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine), Rat (Rattus)
92
8
6
1
1
1
1
1
1
1
Host
Rabbit
55
21
15
Clonality
Polyclonal
Conjugate
Un-conjugated
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
Application
ELISA, Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunoprecipitation (IP), Western Blotting (WB)
70
27
20
19
12
11
6
5
4
3
2
2
2
1
1
1
1
Supplier
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Immunogen Synthetic peptide from N terminal part of human PADI4 protein
Isotype IgG
Specificity Reacts with PADI4 from human, mouse and rat. No cross reaction with the other isoforms.
Purification Purified (protein A)
Alternative Name Peptidylarginine Deiminase Type IV (PAD-4) (PADI4 Antibody Abstract)
UniProt Q9UM07
Application Notes

Working dilution: Optimal dilution should be determined by the end user.
The following are guidelines only :
ELISA(1:1000 - 1:5000) IHC(1:50 - 1:100) IP(1:20 - 1:100) WB(1:200 - 1:2000)

Restrictions For Research Use only
Format Lyophilized
Reconstitution Must be reconstituted in distilled water.
Concentration 1 mg/mL
Buffer Tris 0,1M, glycine 0,1M, sucrose 2 %
Storage 4 °C,-20 °C
Storage Comment Lyophilized powder stable for a minimum of 2 years at -20°C. Store reconstituted antibodies at +4°C. For extended periods store in aliquots at -20°C. Antibodies are guaranteed for 6 month from date of receipt.
Expiry Date 24 months
Background publications Wang, Wysocka, Sayegh et al.: "Human PAD4 regulates histone arginine methylation levels via demethylimination." in: Science (New York, N.Y.), Vol. 306, Issue 5694, pp. 279-83, 2004 (PubMed).

Nakashima, Hagiwara, Ishigami et al.: "Molecular characterization of peptidylarginine deiminase in HL-60 cells induced by retinoic acid and 1alpha,25-dihydroxyvitamin D(3)." in: The Journal of biological chemistry, Vol. 274, Issue 39, pp. 27786-92, 1999 (PubMed).