Peptidyl Arginine Deiminase, Type IV (PADI4) (C-Term) antibody

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Antigen
Synonyms PAD, PAD4, PADI5, PDI4, PDI5, Pad4, Pdi4
Epitope
C-Term
(1)
Reactivity
Human, Mouse (Murine), Rat (Rattus)
(1), (1), (1)
Host
Rabbit
(1)
Clonality
Polyclonal
Application
ELISA, Immunoprecipitation (IP), Immunohistochemistry (IHC), Western Blotting (WB)
(1), (1), (1), (1)
Pubmed 2 references available
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Quantity 200 μL
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Immunogen A15 residue synthetic peptide derived from C-terminal domain of human PADI4 protein
Isotype IgG
Specificity Reacts with 74 kDa PADI4 protein from human, mouse and rat. No cross reaction with the other isoforms.
Cross-Reactivity (Details) Reacts with 74 kDa PADI4 protein from human, mouse and rat. No cross reaction with the other isoforms.
Purification Antiserum
Background PADI4 is the enzyme that catalyze the citrullination or the deimination of arginine on proteins like Histone H3.
Application Notes Optimal dilution should be determined by the end user.
The following are guidelines only :
ELISA 1:1000 - 1:5000 IHC 1:50 - 1:100 IP 1:20 - 1:100 WB 1:200 - 1:2000
Restrictions For Research Use only
Format Lyophilized
Reconstitution Must be reconstituted in distilled water.
Storage -20 °C,4 °C
Storage Comment Lyophilized powder stable for a minimum of 2 years at -20°C. Store reconstituted antibodies at +4°C. For extended periods store in aliquots at -20°C. Antibodies are guaranteed for 6 month from date of receipt.
Expiry Date 24 months
Background publications Wang, Wysocka, Sayegh et al.: "Human PAD4 regulates histone arginine methylation levels via demethylimination." in: Science (New York, N.Y.), Vol. 306, Issue 5694, pp. 279-83, 2004 (PubMed).

Nakashima, Hagiwara, Ishigami et al.: "Molecular characterization of peptidylarginine deiminase in HL-60 cells induced by retinoic acid and 1alpha,25-dihydroxyvitamin D(3)." in: The Journal of biological chemistry, Vol. 274, Issue 39, pp. 27786-92, 1999 (PubMed).

Catalog No. ABIN347070
$426.07
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