Acetylated Lysine antibody

Antigen: Acetylated Lysine

Clonality: Monoclonal (7F8)

Host: Mouse

Reactivity: Bird (Avian), Mammalian

Application: ELISA, Western Blotting (WB), Immunohistochemistry (IHC)

Catalog no.: ABIN349602

Additional Information

Immunogen: This Protein G purified monoclonal antibody was prepared using conventional hybridoma technology after repeated immunizations with acetylated keyhole limpet hemocyanine (KLH). Hybridoma: Produced by the fusion between mouse splenocytes and mouse myeloma SP2/0 cells using conventional hybridoma technology.

Format: Liquid (sterile filtered)

Isotype: IgG1  (Matching secondary antibodies)

Clone: 7F8

Description: Post-translational modifications of proteins play critical roles in the regulation and function of many known biological processes. A c ommon posttranscriptional modification of lysine residues involves acetylation (1). The conserved amino-terminal domains of the four core histones (H2A, H2B, H3 and H4) contain lysines that are acetylated by histone acetyltransferases (HATs) and deacetylated by histone deacetylases (HDACs) (2). Protein posttranslational reversible lysine N ε -acetylation and deacetylation have been recognized as an emerging intracellular signaling mechanism that plays critical roles in regulating gene transcription, cell-cycle progression, apoptosis, DNA repair, and cytoskeletal organization (3). The regulation of protein acetylation status is impaired in the pathologies of cancer and polyglutamine diseases (4). HDACs therefore are promising targets for anticancer drugs currently in development (5).

Specificity: This Protein G purified monoclonal antibody reacts with proteins containing acetylated lysine residues and does not detect non-acetylated lysine residues. This antibody is known to react with acetylated lysine residues from mammalian and avian sources. Reactivity with acetylated lysines from other sources is also expected.

Application Details

Application Notes: Recommended Dilutions: ELISA User Optimized WESTERN BLOT 1:500 - 1:2,000 IMMUNOHISTOCHEMISTRY 1:200 - 1:1,000 OTHER APPLICATIONS User Optimized. Note: This Protein G purified antibody has been tested for use in ELISA, immunohistochemistry and western blotting. Specific conditions for reactivity should be optimized by the end user. Approximately 1 ug of antibody is sufficient to detect 20 ug of acetylated chicken erythrocyte histones (sodium butyrate treated) by Western blotting using HRP Gt-a-Mouse IgG (p/n 610-103-121) and chemiluminescent substrate (p/n PICOMAX-110).

Concentration: 1.0 mg/ml (by UV absorbance at 280 nm)

Buffer: Stabilizer: 50% (v/v) Glycerol. Preservative: 0.09% (w/v) Sodium Azide. Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2.

Storage: Store vial at -20o C or below prior to opening. Dilute only prior to immediate use. For extended storage, aliquot contents and freeze at -20o C or below. Avoid cycles of freezing and thawing. Expiration date is one (1) year from date of opening.

Restrictions: For Research Use only