Nitrotyrosine antibody

Antigen: Nitrotyrosine

Clonality: Monoclonal (39B6)

Host: Mouse

Application: ELISA, Western Blotting (WB), Immunohistochemistry (IHC)

Catalog no.: ABIN349603

Additional Information

Immunogen: This Protein G purified monoclonal antibody was prepared using conventional hybridoma technology after repeated immunizations with 3-(4-Hydroxy-3-nitrophenyl acetamido) propionic acid - BSA conjugate. Hybridoma: Produced by the fusion between mouse splenocytes and mouse myeloma SP2/0 cells using conventional hybridoma technology.

Format: Liquid (sterile filtered)

Isotype: IgG2a  (Matching secondary antibodies)

Clone: 39B6

Description: Protein tyrosine nitration results in a post-translational modification that is increasingly receiving attention as an important component of nitric oxide signaling (2). While multiple nonenzymatic mechanisms are known to be capable of producing nitrated tyrosine residues, most tyrosine nitration events involve catalysis by metalloproteins such as myeloperoxidase, eosinophilperoxidase (3), myoglobin, the cytochrome P-450s, superoxide dismutase and prostacyclin synthase. Nitrotyrosine may also serve as a biomarker for the effects of reactive nitrogen oxides, based on tyrosine residues becoming nitrated in proteins at sites of inflammation-induced tissue injury (1). The presence of nitro tyrosine-containing proteins has shown high correlation to disease states such as atherosclerosis, Alzheimers disease, Parkinsons disease and amyotrophic lateral sclerosis (4).

Specificity: This Protein G purified monoclonal antibody reacts with proteins containing 3-nitrotyrosine moieties and does not detect non-nitrated tyrosine residues. This antibody is known to react with nitrated tyrosine residues from human, mouse, rat and dog sources. Reactivity with nitrotyrosine from other sources is also expected.

Application Details

Application Notes: Recommended Dilutions: ELISA User Optimized WESTERN BLOT 1:500 - 1:2,000 IMMUNOHISTOCHEMISTRY 1:200 - 1:1,000 OTHER APPLICATIONS User Optimized. Note: This Protein G purified antibody has been tested for use in ELISA, immunoprecipitation, immunohistochemistry and western blotting. Specific conditions for reactivity should be optimized by the end user. Approximately 0.7 ug of antibody is sufficient to detect 5 ug of SIN-1 treated BSA by Western blotting using HRP Gt-a-Mouse IgG (p/n 610-103-121) and chemiluminescent substrate (p/n PICOMAX-110).

Concentration: 1.0 mg/ml (by UV absorbance at 280 nm)

Buffer: Stabilizer: 50% (v/v) Glycerol. Preservative: 0.09% (w/v) Sodium Azide. Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2.

Storage: Store vial at -20o C or below prior to opening. Dilute only prior to immediate use. For extended storage, aliquot contents and freeze at -20o C or below. Avoid cycles of freezing and thawing. Expiration date is one (1) year from date of opening.

Research Area: Metabolism, Amino Acids

Restrictions: For Research Use only