Vacuolar H+-Pyrophosphatase (V-PPase) antibody

Antigen: Vacuolar H+-Pyrophosphatase (V-PPase)

Clonality: Polyclonal

Host: Rabbit

Reactivity: Arabidopsis thaliana

Application: ELISA, Western Blotting (WB), Immunolocalization (IL)

Catalog no.: ABIN349652

Additional Information

Immunogen: KLH-conjugated synthetic peptide derived from Arabidopsis thaliana V-PPase,P31414

Format: Serum

Description: V-PPase (pyrophosphate-energized vacuolar membrane proton pump 1),EC=3.6.1.1, acts to establish proton gradient of often higher magnitude thanH+-ATPase. Is involved in auxin transport and NaCl and drought tolerance. Alternative names: pyrophosphate-energized inorganic pyrophosphatase 1,H(+)-PPase 1, vacuolar proton pyrophosphatase 1, vacuolar protonpyrophosphatase 3

Specificity: Predicted reactivity: dicots including: Nicotiana tabacum, Ricinus communis, Vitis vinifera, Saccharumofficinarum, monocots including: Horderum vulgare, Oryza sativa, Zea mays,trees: Picea sitchensis, Populus trichocarpa.

Application Details

Application Notes: Recommended Dilution 1: 8000 (ELISA), 1: 2000 with standard ECL (WB), 1: 100 (IL).

Buffer: 0.1 % sodium azide is added as preservative. For antibody re-suspendinginformation check the tube lable. Antibodies will detect target protein in a few µg of a crude preparation loaded perwell. If purified preparations of vacuolar and plasma membranes are

Storage: store at -20°C, make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin tubes briefly prior to opening them to avoid any losses thatmight occur from material adhering to the cap or sides of the tubes.

Research Area: Plant/Algal Physiology

Restrictions: For Research Use only

Images

1ug and 10ug of crude membrane fraction/lane from Arabidopsis thaliana wereseparated on 12 % SDS-PAGE and blotted 1h to PVDF membrane (40 min. at 10 V usingBioRad semidry transfer). Filters were blocked 1h with 5 % low-fat milk powder in TBS-T(0.05% Triton X.100). Membranes were washed 5 times with TBS-T, each time in a freshpolystyrene box and probed with anti-V-PPase antibodies (ABIN349652, 1:2000, 1h) andsecondary anti-rabbit (1:2000, 1 h). All steps were performed in RT with agitation.

Publications

Publications: Kobae, Mizutani, Segami et al.: "Immunochemical analysis of aquaporin isoforms in Arabidopsis suspension-cultured cells." in: Bioscience, biotechnology, and biochemistry, Vol. 70, Issue 4, pp. 980-7, 2006 (PubMed).

Kabaa, Janicka-Russak: "Differential regulation of vacuolar H+-ATPase and H+-PPase in Cucumis sativus roots by zinc and nickel." in: Plant science : an international journal of experimental plant biology, Vol. 180, Issue 3, pp. 531-9, 2011 (PubMed).