ATPase, H+ Transporting, Lysosomal V1 Subunit C (ATP6V1C) antibody

Antigen: ATPase, H+ Transporting, Lysosomal V1 Subunit C (ATP6V1C)

Clonality: Polyclonal

Host: Rabbit

Reactivity: Arabidopsis thaliana, Raphanus sativus

Application: ELISA, Western Blotting (WB)

Catalog no.: ABIN349655

Additional Information

Alternative name: vacuolar H+-ATPase, subunit c (16 kDa) (V-ATPase, c)

Immunogen: KLH-conjugated synthetic peptide derived from Arabidopsis thaliana V-ATPase subunit c,Q6IDA4

Format: Liquid

Description: V-ATPase c subunit is located in vacuole and is involved in ATP synthesis cuopled proton transport. This protein is coded by ATVHA-C3 gene. Alternative names: AT4g34720/T4L20_300

Specificity: Predicted reactivity: dictos including: Gossypium mexicanum, Phaseolus aureus, Rcicinus communis, monocots including: Oryza sativa, Triticum aestivum, Zea mays, trees: Picea sitchensis, Populus trichocarpa. Not reactive in: no confirmed exceptions from predicted reactivity known in the moment.

Application Details

Application Notes: Recommended dilution: 1: 8000 (ELISA), 1: 2000 with standard ECL (WB). Molecular Weight: 16 | 16 kDa (Arabidopsis thaliana) (expected, apparent). Additional information: Protein or membrane sample should be treated at 70oC for 10 min before loading on the gel.Diluted antibody solution can be used 2 to 3 times within one month if it contains 0.1 % sodium azide as preservative and is stored at -20oC to -80oC. Manufactured by Operon Biotechnologies.

Buffer: serum

Storage: store lyophilized/reconstituted at -20°C, once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior toopening them to avoid any losses that might occur from lyophilized materialadhering to the cap or sides of the tubes.

Research Area: Plant/Algal Physiology

Restrictions: For Research Use only

Images

1ug, 5 and 10ug of vacuolar membrane fraction/lane from Raphanus sativus were separated on 12 % SDS-PAGE and blotted 1h to PVDF membrane (40 min. at 10 V usingBioRad semidry transfer). Filters were blocked 1h with 5 % low-fat milk powder in TBS-T(0.05% Triton X.100). Membranes were washed 5 times with TBS-T, each time in a freshpolystyrene box and probed with anti-V-ATPase c subunit antibodies (ABIN349655, 1:1000,1h) and secondary anti-rabbit (1:2000, 1 h). All steps were performed in RT with agitation.

anti-ATPase, H+ Transporting, Lysosomal V1 Subunit C (ATP6V1C) antibody (Image 2)

Publications

Publications: Kobae, Mizutani, Segami et al.: "Immunochemical analysis of aquaporin isoforms in Arabidopsis suspension-cultured cells." in: Bioscience, biotechnology, and biochemistry, Vol. 70, Issue 4, pp. 980-7, 2006 (PubMed).