Plasma Membrane Aquaporin (PIP2-7) antibody

Antigen: Plasma Membrane Aquaporin (PIP2-7)

Clonality: Polyclonal

Host: Rabbit

Reactivity: Arabidopsis thaliana

Application: ELISA, Western Blotting (WB)

Catalog no.: ABIN349656

Additional Information

Immunogen: KLH-conjugated synthetic peptide derived from Arabidopsis thalianaPIP2,7,P93004

Format: Serum

Description: Plasma membrane aquaporin, PIP2,7 is water channel protein required forwater transport across cell membrane. Alternative names: plasma membraneintrinsic protein 2-7, AtPIP2,7, plasma membrane intrinsic protein 3, saltstress-induced major intrinsic protein, PIP3a

Specificity: Predicted reactivity: dictos including: Brassica oleracea, Spinacia oleracea, Vitis vinifera,trees: Juglansregia, Populus tremulax Populus tremuloides.

Application Details

Application Notes: Recommended Dilution 1: 8000 (ELISA), 1: 2000 with standard ECL (WB).

Buffer: 0.1 % sodium azide is added as preservative. For antibody re-suspendinginformation check the tube lable. Antibodies will detect target protein in a few µg of a crude preparation loaded perwell. If purified preparations of plasma membranes are used, one µg

Storage: store at -20°C, make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin tubes briefly prior to opening them to avoid any losses thatmight occur from material adhering to the cap or sides of the tubes.

Research Area: Plant/Algal Physiology

Restrictions: For Research Use only

Images

1ug and 10ug of crude membrane fraction/lane from Arabidopsis thaliana wereseparated on 12 % SDS-PAGE and blotted 1h to PVDF membrane (40 min. at 10 V usingBioRad semidry transfer). Filters were blocked 1h with 5 % low-fat milk powder in TBS-T(0.05% Triton X.100). Membranes were washed 5 times with TBS-T, each time in a freshpolystyrene box and probed with anti-PIP2,7 antibodies (ABIN349656, 1:1000, 1h) andsecondary anti-rabbit (1:2000, 1 h). All steps were performed in RT with agitation.