ABC Transporter C Family Member 1 (AtABCC1) antibody

Antigen: ABC Transporter C Family Member 1 (AtABCC1)

Clonality: Polyclonal

Host: Rabbit

Reactivity: Arabidopsis thaliana

Application: ELISA, Western Blotting (WB)

Catalog no.: ABIN349658

Additional Information

Alternative name: ABC transporter C family member 1 (AtMRP1, AtABCC1)

Immunogen: KLH-conjugated synthetic peptide derived from Arabidopsis thalianaMRP1,Q9C8G9

Format: Serum

Description: MRP1 (EC= 3.6.3.44) is a pump for glutathione S-conjugates. Alternative names:ABC transporter ABCC.1, multidrug resistance-associated protein 1, glutathioneS-conjugate-transporting ATPase 1, ATP-energized glutathione S-conjugatepump 1

Specificity: Predicted reactivity: dicots including: Vitis vinifera, monocots including: Oryza sativa, Triticumaestivum, trees: Populus balsamifera, moss: Physcomitrella patens.

Application Details

Application Notes: Recommended Dilution 1: 8000 (ELISA), 1: 2000 with standard ECL (WB).

Buffer: 0.1 % sodium azide is added as preservative. For antibody re-suspendinginformation check the tube lable. Antibodies will detect target protein in a few µg of a crude preparation loaded perwell. If purified preparations of vacuolar and plasma membranes are

Storage: store at -20°C, make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin tubes briefly prior to opening them to avoid any losses thatmight occur from material adhering to the cap or sides of the tubes.

Research Area: Plant/Algal Physiology

Restrictions: For Research Use only

Images

1ug and 10ug of crude membrane fraction/lane from Arabidopsis thaliana were separated on12 % SDS-PAGE and blotted 1h to PVDF membrane (40 min. at 10 V using BioRad semidrytransfer). Filters were blocked 1h with 5 % low-fat milk powder in TBS-T (0.05% Triton X.100).Membranes were washed 5 times with TBS-T, each time in a fresh polystyrene box and probedwith anti-AtMTP4 antibodies (ABIN349658, 1:1000, 1h) and secondary anti-rabbit (1:2000, 1 h). Allsteps were performed in RT with agitation.

anti-ABC Transporter C Family Member 1 (AtABCC1) antibody (Image 2)

Publications

Publications: Geisler, Girin, Brandt et al.: "Arabidopsis immunophilin-like TWD1 functionally interacts with vacuolar ABC transporters." in: Molecular biology of the cell, Vol. 15, Issue 7, pp. 3393-405, 2004 (PubMed).