Nitrate Transporter (AJ011604) antibody

Antigen: Nitrate Transporter (AJ011604)

Clonality: Polyclonal

Host: Rabbit

Reactivity: Arabidopsis thaliana, Raphanus sativus

Application: ELISA, Western Blotting (WB)

Catalog no.: ABIN349659

Additional Information

Immunogen: KLH-conjugated synthetic peptide derived from Arabidopsis thaliana nitratetransporter Q9SZY4

Format: Serum

Description: Nitrate transporter functions in transporter activity in response to jasmonic acidstimulus and response to wounding. This protein is coded by ntp2 gene.

Specificity: Predicted reactivity: Arabidopsis thaliana, Raphanus sativus.

Application Details

Application Notes: Recommended Dilution 1: 8000 (ELISA), 1: 1000 with standard ECL (WB).

Buffer: 0.1 % sodium azide is added as preservative. For antibody re-suspendinginformation check the tube lable. This protein is present in plant tissues in very low levels. Protocol for isolation of plant vacuolar membranes can be found here.

Storage: store at -20°C, make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin tubes briefly prior to opening them to avoid any losses thatmight occur from material adhering to the cap or sides of the tubes.

Research Area: Plant/Algal Physiology

Restrictions: For Research Use only

Images

5ug and 10ug of vacuolar membrane fraction/lane from Raphanus sativus wereseparated on 12 % SDS-PAGE and blotted 1h to PVDF membrane (40 min. at 10 V usingBioRad semidry transfer). Filters were blocked 1h with 5 % low-fat milk powder in TBS-T(0.05% Triton X.100). Membranes were washed 5 times with TBS-T, each time in a freshpolystyrene box and probed with anti-AJ011604 | nitrate transporter antibodies (ABIN349659,1:1000, 1h) and secondary anti-rabbit (1:2000, 1 h). All steps were performed in RT withagitation.