40S ribosomal protein S14-1 (S14) antibody

Antigen: 40S ribosomal protein S14-1 (S14)

Clonality: Polyclonal

Host: Rabbit

Reactivity: Arabidopsis thaliana

Application: ELISA, Western Blotting (WB)

Catalog no.: ABIN349663

Additional Information

Immunogen: KLH-conjugated synthetic peptide derived from Arabidopsis thaliana Q9SIH0

Format: Serum

Description: 40S ribosomal protein S14-1 is localized in cytoplasm and belongs to ribosomalprotein S11P family.

Specificity: Predicted reactivity: dicots including: Brassica napus, Lupinus luteus, Ricinus communis, monocotsincluding: Oryza sativa, Zea mays, trees: Picea sitchensis, Populus trichocarpa,algae: Chlamydomonas reinhardii, microalgae: Ostreococcus tauri other species: chicken, human, mouse, rat, salmon, Candidia albicans,Trypanosoma brucei.

Application Details

Application Notes: Recommended Dilution 1: 8000 (ELISA), 1: 1000 with standard ECL (WB).

Buffer: 0.1 % sodium azide is added as preservative. For antibody re-suspendinginformation check the tube lable. Antibodies will detect target protein in a few µg of a crude preparation loaded perwell. If purified preparations of vacuolar and plasma membranes are

Storage: store at -20°C, make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin tubes briefly prior to opening them to avoid any losses thatmight occur from material adhering to the cap or sides of the tubes.

Research Area: Plant/Algal Physiology

Restrictions: For Research Use only

Images

1ug and 10ug of crude membrane fraction/lane from Arabidopsis thaliana wereseparated on 12 % SDS-PAGE and blotted 1h to PVDF membrane (40 min. at 10 V usingBioRad semidry transfer). Filters were blocked 1h with 5 % low-fat milk powder in TBS-T(0.05% Triton X.100). Membranes were washed 5 times with TBS-T, each time in a freshpolystyrene box and probed with anti-S14 antibodies (ABIN349659, 1:1000, 1h) andsecondary anti-rabbit (1:2000, 1 h). All steps were performed in RT with agitation.