60S ribosomal protein L13-1 (L13-1) antibody

Antigen: 60S ribosomal protein L13-1 (L13-1)

Clonality: Polyclonal

Host: Rabbit

Reactivity: Arabidopsis thaliana

Application: ELISA, Western Blotting (WB)

Catalog no.: ABIN349664

Additional Information

Immunogen: KLH-conjugated synthetic peptide derived from Arabidopsis thaliana P41127

Format: Serum

Description: L13 protein is localized in the cytoplasm. It belongs to the ribosomal protein L13efamily. Alternative name: protein BBC1 homolog

Specificity: Predicted reactivity: dicots including: Brassica napus, Nicotiana tabacum, Ricinus communis, Vitisvinifera, monocots including: Oryza sativa, Zea mays, trees: Picea excelsa,Populus balsamifera, algae: Chlamydomonas reinhardii.

Application Details

Application Notes: Recommended Dilution 1: 8000 (ELISA), 1: 1000 with standard ECL (WB).

Buffer: 0.1 % sodium azide is added as preservative. For antibody re-suspendinginformation check the tube lable. Antibodies will detect target protein in a few µg of a crude preparation loaded perwell. If purified preparations of vacuolar and plasma membranes are

Storage: store at -20°C, make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin tubes briefly prior to opening them to avoid any losses thatmight occur from material adhering to the cap or sides of the tubes.

Research Area: Plant/Algal Physiology

Restrictions: For Research Use only

Images

1ug and 10ug of crude membrane fraction/lane from Arabidopsis thaliana wereseparated on 12 % SDS-PAGE and blotted 1h to PVDF membrane (40 min. at 10 V usingBioRad semidry transfer). Filters were blocked 1h with 5 % low-fat milk powder in TBS-T(0.05% Triton X.100). Membranes were washed 5 times with TBS-T, each time in a freshpolystyrene box and probed with anti-L13 antibodies (ABIN349664, 1:1000, 1h) and secondaryanti-rabbit (1:2000, 1 h). All steps were performed in RT with agitation.