gamma TIP (AtTIP1,1) antibody

Antigen: gamma TIP (AtTIP1,1)

Clonality: Polyclonal

Host: Rabbit

Reactivity: Arabidopsis thaliana

Application: ELISA, Western Blotting (WB)

Catalog no.: ABIN349666

Additional Information

Immunogen: KLH-conjugated synthetic peptide derived from Arabidopsis thaliana P25818

Format: Serum

Description: TIP1,1 is a water channel protein needed for the transport of water, diffusion ofamino acids and peptides. Alternative name: Tonoplast intrinsic protein 1-1,gamma-tonoplast intrinsic protein, Gamma-TIP, aquaporin TIP, Tonoplast intrinsicprotein, root-specific RB7

Specificity: Predicted reactivity: dictos including: Brassica oleracea, Gossypium mexicanum, Pisum sativum,Ricinus communis, Vitis vinifera, monocots including: Oryza sativa, trees: Piceaabies, Popoulus trichocarpa.

Application Details

Application Notes: Recommended Dilution 1: 8000 (ELISA), 1: 2000 with standard ECL (WB).

Buffer: 0.1 % sodium azide is added as preservative. For antibody re-suspendinginformation check the tube lable. Antibodies will detect target protein in a few µg of a crude preparation loaded perwell. If purified preparations of vacuolar and plasma membranes are

Storage: store at -20°C, make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin tubes briefly prior to opening them to avoid any losses thatmight occur from material adhering to the cap or sides of the tubes.

Research Area: Plant/Algal Physiology

Restrictions: For Research Use only

Images

1ug and 10ug of crude membrane fraction/lane from Arabidopsis thaliana wereseparated on 12 % SDS-PAGE and blotted 1h to PVDF membrane (40 min. at 10 V usingBioRad semidry transfer). Filters were blocked 1h with 5 % low-fat milk powder in TBS-T(0.05% Triton X.100). Membranes were washed 5 times with TBS-T, each time in a freshpolystyrene box and probed with anti-TIP1,1 antibodies (ABIN349666, 1:1000, 1h) andsecondary anti-rabbit (1:2000, 1 h). All steps were performed in RT with agitation. TIP1,1 usually shows a faint band of dimeric form at 42 kDa in addition to the majormonomoer band of 23 kDa even in the presence of SDS.

Publications

Publications: Ishikawa, Suga, Uemura et al.: "Novel type aquaporin SIPs are mainly localized to the ER membrane and show cell-specific expression in Arabidopsis thaliana." in: FEBS letters, Vol. 579, Issue 25, pp. 5814-20, 2005 (PubMed).