Vacuolar Zn2+/H+ Antiporter (AtMTP1) antibody

Antigen: Vacuolar Zn2+/H+ Antiporter (AtMTP1)

Clonality: Polyclonal

Host: Rabbit

Reactivity: Arabidopsis thaliana

Application: ELISA, Western Blotting (WB)

Catalog no.: ABIN349669

Additional Information

Immunogen: KLH-conjugated synthetic peptide derived from Arabidopsis thaliana MTP1Q9ZT63

Format: Serum

Description: MTP1 is mediating zinc accumulation in roots and resistance to zinc. Is involved insequestering of zinc from cytoplasm into vacuole in conditions of its excess.Alternative names: Zinc transporter ZAT-1, ZAT1p, 43.8 | metal tolerance protein1

Specificity: Predicted reactivity: dicots including: Brassica sp. , Noccaea fendleri, Thlaspi caerulescens.

Application Details

Application Notes: Recommended Dilution 1: 8000 (ELISA), 1: 1000 with standard ECL (WB).

Buffer: 0.1 % sodium azide is added as preservative. For antibody re-suspendinginformation check the tube lable. MTP1 protein is of low abundance in plant tissues. Protocol for isolation of tonoplast proteins can be found here.

Storage: store at -20°C, make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin tubes briefly prior to opening them to avoid any losses thatmight occur from material adhering to the cap or sides of the tubes.

Research Area: Plant/Algal Physiology

Restrictions: For Research Use only

Images

sample of a vector (1) and vacuolar membrane fraction of yeast cells expressing AtMTP1 (2)separated on 12 % SDS-PAGE and blotted 1h to PVDF membrane (40 min. at 10 V usingBioRad semidry transfer). Filters were blocked 1h with 5 % low-fat milk powder in TBS-T(0.05% Triton X.100). Membranes were washed 5 times with TBS-T, each time in a freshpolystyrene box and probed with anti-AtMTP1 antibodies (ABIN349669, 1:1000, 1h) andsecondary anti-rabbit (1:2000, 1 h). All steps were performed in RT with agitation.

Publications

Publications: Kobae, Uemura, Sato et al.: "Zinc transporter of Arabidopsis thaliana AtMTP1 is localized to vacuolar membranes and implicated in zinc homeostasis." in: Plant & cell physiology, Vol. 45, Issue 12, pp. 1749-58, 2005 (PubMed).

Kawachi, Kobae, Mimura et al.: "Deletion of a histidine-rich loop of AtMTP1, a vacuolar Zn(2+)/H(+) antiporter of Arabidopsis thaliana, stimulates the transport activity." in: The Journal of biological chemistry, Vol. 283, Issue 13, pp. 8374-83, 2008 (PubMed).