PIP1,1/PIP1,2/PIP1,3 antibody

Catalog No. ABIN349671

Product Details anti-PIP1,1/PIP1,2/PIP1,3 Antibody

Target: PIP1,1/PIP1,2/PIP1,3 (Aquaporins (PIP1,1, PIP1,2, PIP1,3) (PIP1,1/PIP1,2/PIP1,3))

Reactivity: Arabidopsis thaliana

Host: Rabbit

Clonality: Polyclonal

Application: ELISA, Western Blotting (WB)

Characteristics: Expected / apparent Molecular Weight of the Antigene: 30.68 / 28 kDa

Purification: serum

Immunogen: synthetic peptideconserved in Arabidopsis thaliana: PIP1,1 P61837,At3g61430 PIP1,2 Q06611,At3g45960 PIP1,3 Q08733,At3g01620 , PIP1,4 Q39196, At3g00430, PIP1,5 Q8LAA6 At3g23400

Application Details

Application Notes: Recommended Dilution: 1 : 8000 (ELISA), 1 : 1000 with standard ECL (WB).
Antibodies will detect target protein in a few µg of a crude preparation loaded per well. If purified preparations of vacuolar and plasma membranes are used, one µg load per well should be sufficient.

Comment:

Protein or membrane sample should be treated at 70°C for 10 min before loading on the gel.Diluted antibody solution can be used 2 to 3 times within one month if it contains 0.1 % sodium azide as preservative and is stored at -20°C to -80°C.Manufactured by Operon Biotechnologies.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: 0.1 % sodium azide is added as preservative

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tubes.

Storage: -20 °C

Target Details for PIP1,1/PIP1,2/PIP1,3

Target: PIP1,1/PIP1,2/PIP1,3 (Aquaporins (PIP1,1, PIP1,2, PIP1,3) (PIP1,1/PIP1,2/PIP1,3))

Alternative Name: PIP1 (PIP1,1, PIP1,2, PIP1,3) aquaporins

Background: AGI Code: At3g61430
PIPs proteins are aquaporins which facilitate the transport of water and small neutral molecules across cell membrane. Alternative names: PIP1-1, PIP1-2, PIP1-3, plasma membrane intrinistic protein 1a, 1b, 1c, PIP1a, PIP1b, PIP1c, plasma membrane aquaporin-1, transmembrane protein A, TMP-A, AthH2, transmembrane protein B, TMP-B,

Molecular Weight: expected: 30.68 kDa, apparent: 28 kDa

UniProt: Q39196, Q06611, Q08733