Aquaporin PIP2,1 (PIP2,1) antibody

Antigen: Aquaporin PIP2,1 (PIP2,1)

Clonality: Polyclonal

Host: Rabbit

Reactivity: Arabidopsis thaliana, Raphanus sativus

Application: ELISA, Western Blotting (WB)

Catalog no.: ABIN349672

Additional Information

Immunogen: KLH-conjugated synthetic peptide derived from Raphanus sativus PAQ2 O80369

Format: Serum

Description: PIPs proteins are aquaporins which facilitate the transport of water and smallneutral molecules across cell membrane.

Specificity: Predicted reactivity: dicots including: Brassica napus, Gossypium mexicanum.

Application Details

Application Notes: Recommended Dilution 1: 8000 (ELISA), 1: 1000 with standard ECL (WB).

Buffer: 0.1 % sodium azide is added as preservative. For antibody re-suspendinginformation check the tube lable. Antibodies will detect target protein in a few µg of a crude preparation loaded perwell. If purified preparations of vacuolar and plasma membranes are

Storage: store at -20°C, make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin tubes briefly prior to opening them to avoid any losses thatmight occur from material adhering to the cap or sides of the tubes.

Research Area: Plant/Algal Physiology

Restrictions: For Research Use only

Images

1ug and 10ug of crude membrane fraction/lane from Arabidopsis thaliana wereseparated on 12 % SDS-PAGE and blotted 1h to PVDF membrane (40 min. at 10 V usingBioRad semidry transfer). Filters were blocked 1h with 5 % low-fat milk powder in TBS-T(0.05% Triton X.100). Membranes were washed 5 times with TBS-T, each time in a freshpolystyrene box and probed with anti-PIP2,1 antibodies (ABIN349672, 1:1000, 1h) andsecondary anti-rabbit (1:2000, 1 h). All steps were performed in RT with agitation.

Publications

Publications: Geisler, Girin, Brandt et al.: "Arabidopsis immunophilin-like TWD1 functionally interacts with vacuolar ABC transporters." in: Molecular biology of the cell, Vol. 15, Issue 7, pp. 3393-405, 2004 (PubMed).