Aquaporins (PIP1,1, PIP1,2, PIP1,3) (PIP1,1/PIP1,2/PIP1,3) antibody

Antigen: Aquaporins (PIP1,1, PIP1,2, PIP1,3) (PIP1,1/PIP1,2/PIP1,3)

Clonality: Polyclonal

Host: Rabbit

Reactivity: Arabidopsis thaliana, Raphanus sativus

Application: ELISA, Western Blotting (WB)

Catalog no.: ABIN349673

Additional Information

Alternative name: Aquaporins (PIP1,1, PIP1,2, PIP1,3)

Immunogen: KLH-conjugated synthetic peptide derived from N terminus of Raphanussativus PAQ1O80368. Chosen peptide is conserved in PIP1,1, PIP1,2, PIP1,3N-terminus.

Format: Serum

Description: PIPs proteins are aquaporins which facilitate the transport of water and smallneutral molecules across cell membrane.

Specificity: Predicted reactivity: dicots including: Lycopersicum esculentum, Ricinus communis, monocotsincluding: Hordeum vulgare, Oryza sativa, Zea mays, trees: Populus trichocarpa.

Application Details

Application Notes: Recommended Dilution 1: 8000 (ELISA), 1: 1000 with standard ECL (WB).

Buffer: 0.1 % sodium azide is added as preservative. For antibody re-suspendinginformation check the tube lable. Antibodies will detect target protein in a few µg of a crude preparation loaded perwell. If purified preparations of vacuolar and plasma membranes are

Storage: store at -20°C, make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin tubes briefly prior to opening them to avoid any losses thatmight occur from material adhering to the cap or sides of the tubes.

Research Area: Plant/Algal Physiology

Restrictions: For Research Use only

Images

1ug and 10ug of crude membrane fraction/lane from Arabidopsis thaliana wereseparated on 12 % SDS-PAGE and blotted 1h to PVDF membrane (40 min. at 10 Vusing BioRad semidry transfer). Filters were blocked 1h with 5 % low-fat milk powderin TBS-T (0.05% Triton X.100). Membranes were washed 5 times with TBS-T, eachtime in a fresh polystyrene box and probed with anti-PIP1s ntibodies (diluted in 1: 500(left panel) and 1: 1000 (right panel), ABIN349673, and secondary anti-rabbit (1:2000, 1h). All steps were performed in RT with agitation.