Plasma Membrane Aquaporin 2b (PIP2,2) antibody

Antigen: Plasma Membrane Aquaporin 2b (PIP2,2)

Clonality: Polyclonal

Host: Rabbit

Reactivity: Arabidopsis thaliana, Raphanus sativus

Application: ELISA, Western Blotting (WB)

Catalog no.: ABIN349674

Additional Information

Immunogen: KLH-conjugated synthetic peptide derived from Raphanus sativus PAQ2bQ9MB99

Format: Serum

Description: PIP2,2 is a plasma membrane aquaporin.

Specificity: Predicted reactivity: dicots including: Brassica sp.

Application Details

Application Notes: Recommended Dilution 1: 8000 (ELISA), 1: 2000 with standard ECL (WB).

Buffer: 0.1 % sodium azide is added as preservative. For antibody re-suspendinginformation check the tube lable. Antibodies will detect target protein in a few µg of a crude preparation loaded perwell. If purified preparations of vacuolar and plasma membranes are

Storage: store at -20°C, make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin tubes briefly prior to opening them to avoid any losses thatmight occur from material adhering to the cap or sides of the tubes.

Research Area: Plant/Algal Physiology

Restrictions: For Research Use only

Images

1ug and 10ug of crude membrane fraction/lane from Arabidopsis thaliana wereseparated on 12 % SDS-PAGE and blotted 1h to PVDF membrane (40 min. at 10 V usingBioRad semidry transfer). Filters were blocked 1h with 5 % low-fat milk powder in TBS-T(0.05% Triton X.100). Membranes were washed 5 times with TBS-T, each time in a freshpolystyrene box and probed with anti-PIP2,2 antibodies (ABIN349674, 1:1000, 1h) andsecondary anti-rabbit (1:2000, 1 h). All steps were performed in RT with agitation. PIP proteins usually show a faint band of dimeric form at 55 kDa addition to the majormonomer band of 28 kDa even in the presence of SDS.