Plasma Membrane Intrinistic Protein 2-1,2-2, 2-3 antibody

Antigen: Plasma Membrane Intrinistic Protein 2-1,2-2, 2-3

Clonality: Polyclonal

Host: Rabbit

Reactivity: Arabidopsis thaliana, Raphanus sativus

Application: ELISA, Western Blotting (WB)

Catalog no.: ABIN349675

Additional Information

Alternative name: Plasma membrane intrinistic protein 2-1,2-2, 2-3 (PIP2,1,PIP2,2,PIP2,3)

Immunogen: KLH-conjugated synthetic peptide derived from Arabidopsis thaliana PIP2proteins: AtPIP2-1, AtPIP2-2 P43287, AtPIP2-3 P30302

Format: Serum

Description: PIP2,2 is a plasma membrane aquaporin. Alternative names of isoforms:aquaporin PIP2-1, plasma membrane intrinsic protein 2a, PIP2a, aquaporinPIP2-2, plasma membrane intrinsic protein 2b, PIP2b, TMP2b, Aquaporin PIP2-3,plasma membrane intrinsic protein 2c, PIP2c, TMP2C, RD28-PIP, waterstress-induced tonoplast intrinsic protein, (WSII-TIP)

Specificity: Predicted reactivity: dicots including: Brassica napus, Gossypium hirsutum, Ricinus communis,monocots including: Hordeum vulgare Oryza sativa, Zea mays, trees: Populustremula x Populus tremloides, moss: Physcomitrella patens.

Application Details

Application Notes: Recommended Dilution 1: 8000 (ELISA), 1: 1000 with standard ECL (WB).

Buffer: 0.1 % sodium azide is added as preservative. For antibody re-suspendinginformation check the tube lable. Antibodies will detect target protein in a few µg of a crude preparation loaded perwell. If purified preparations of vacuolar and plasma membranes are

Storage: store at -20°C, make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin tubes briefly prior to opening them to avoid any losses thatmight occur from material adhering to the cap or sides of the tubes.

Research Area: Plant/Algal Physiology

Restrictions: For Research Use only

Images

1ug and 10ug of crude membrane fraction/lane from Arabidopsis thaliana wereseparated on 12 % SDS-PAGE and blotted 1h to PVDF membrane (40 min. at 10 V usingBioRad semidry transfer). Filters were blocked 1h with 5 % low-fat milk powder in TBS-T(0.05% Triton X.100). Membranes were washed 5 times with TBS-T, each time in a freshpolystyrene box and probed with anti-PIP2s antibodies (ABIN349675, 1:1000, 1h) and secondaryanti-rabbit (1:2000, 1 h). All steps were performed in RT with agitation.