Tonoplast Intrinistic Protein 1-1, 1-2 (Gamma) (TIP1,1, TIP1,2) antibody

Antigen: Tonoplast Intrinistic Protein 1-1, 1-2 (Gamma) (TIP1,1, TIP1,2)

Clonality: Polyclonal

Host: Rabbit

Reactivity: Arabidopsis thaliana, Raphanus sativus

Application: ELISA, Western Blotting (WB)

Catalog no.: ABIN349677

Additional Information

Immunogen: KLH-conjugated synthetic peptide conserved in Raphanus sativus TIP1,1 andTIP1,2 (protein accesion number available in Suga et al. 2001). Peptide is alsoconserved in Arabidopsis thaliana TIP1-1 P25818, TIP1-2 Q41963

Format: Serum

Description: TIPs belong to MIP/aquaporin protein family.Alternative names: AquaporinTIP1-1,gamma-tonoplast intrinsic protein, gamma-TIP, aquaporin TIP,gamma-tonoplast intrinsic protein, gamma-TIP, aquaporin TIP, tonoplast intrinsicprotein, root-specific RB7, gamma-tonoplast intrinsic protein 2, gamma-TIP2, saltstress-induced tonoplast intrinsic protein

Specificity: Predicted reactivity: dicots including: Brassica napus, Gossypium hirsutum, Ricinus communis,monocots including: Hordeum vulgare, Oryza sativa, trees: Populus trichocarpa.

Application Details

Application Notes: Recommended Dilution 1: 8000 (ELISA), 1: 1000 with standard ECL (WB).

Buffer: 0.1 % sodium azide is added as preservative. For antibody re-suspendinginformation check the tube lable. Antibodies will detect target protein in a few µg of a crude preparation loaded perwell. If purified preparations of vacuolar and plasma membranes are

Storage: store at -20°C, make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin tubes briefly prior to opening them to avoid any losses thatmight occur from material adhering to the cap or sides of the tubes.

Research Area: Plant/Algal Physiology

Restrictions: For Research Use only

Images

1ug and 10ug of crude membrane fraction/lane from Arabidopsis thaliana wereseparated on 12 % SDS-PAGE and blotted 1h to PVDF membrane (40 min. at 10 V usingBioRad semidry transfer). Filters were blocked 1h with 5 % low-fat milk powder in TBS-T(0.05% Triton X.100). Membranes were washed 5 times with TBS-T, each time in a freshpolystyrene box and probed with anti-TIP1s antibodies (ABIN349659, 1:1000, 1h) andsecondary anti-rabbit (1:2000, 1 h). All steps were performed in RT with agitation. TIP proteins usually show a faint band of dimeric form at 42 kDa in addition to the majormonomer band of 23 kDa even in the presence of SDS.

Publications

Publications: Suga, Imagawa, Maeshima: "Specificity of the accumulation of mRNAs and proteins of the plasma membrane and tonoplast aquaporins in radish organs." in: Planta, Vol. 212, Issue 2, pp. 294-304, 2001 (PubMed).