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Antibody
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anti-Delta-VM23 TIP21 delta
Delta-VM23 (TIP2,1 (delta)) antibody
| Antigen |
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| Clonality |
Polyclonal |
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Host
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Rabbit
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Reactivity
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Raphanus sativus
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Application
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ELISA, Western Blotting (WB)
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| Catalog no. |
ABIN349678 |
| Quantity |
100 ul |
| Price |
638.56 $ Plus shipping costs $35.00
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| Shipping to |
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| Availability |
Ships within 3 to 5 Business days |
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Immunogen
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KLH-synthetic peptide conserved in Raphanus sativus TIP2,1 (delta) O82142
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Format
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Serum
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Description
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TIPs belong to MIP/aquaporin protein family. Alternative name of radish TIP2,1(delata) is delta-VM23.
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Specificity
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Predicted reactivity: dicots: Arabidopsis thaliana.
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Application Notes
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Recommended Dilution 1: 8000 (ELISA), 1: 1000 with standard ECL (WB).
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Buffer
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0.1 % sodium azide is added as preservative. For antibody re-suspendinginformation check the tube lable. Antibodies will detect target protein in a few µg of a crude preparation loaded perwell. If purified preparations of vacuolar and plasma membranes are
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Storage
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store at -20°C, make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin tubes briefly prior to opening them to avoid any losses thatmight occur from material adhering to the cap or sides of the tubes.
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Research Area
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Plant/Algal Physiology
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Restrictions
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For Research Use only
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crude membrane of yeast cells expressing recombinaTIP2,1 (RsTIP2,1) andTIP1,1 (RsTIP1,1) proteins were separated on 12 % SDS-PAGE and blotted 1h toPVDF membrane (40 min. at 10 V using BioRad semidry transfer). Filters wereblocked 1h with 5 % low-fat milk powder in TBS-T (0.05% Triton X.100). Membraneswere washed 5 times with TBS-T, each time in a fresh polystyrene box and probedwith anti-TIP2,1 antibodies (ABIN349678, 1:2000, 1h) and secondary anti-rabbit (1:2000,1 h). All steps were performed in RT with agitation.Left panel: antibody incubacted without peptide, right panel with peptide used to elicitthis antibody (peptide competition assay)
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