Probable Aquaporin SIP2-1 antibody

Antigen: Probable Aquaporin SIP2-1

Clonality: Polyclonal

Host: Rabbit

Reactivity: Arabidopsis thaliana

Application: ELISA, Western Blotting (WB)

Catalog no.: ABIN349680

Additional Information

Alternative name: Probable aquaporin SIP2-1 (AtSIP2,1)

Immunogen: KLH-conjugated synthetic peptide conserved in Arabidopsis thaliana SIP2,1Q9M1K3

Format: Serum

Description: SIP protein functions as a water channel to facilitate the transport of water acrosscell membrane. It is mainly localized to ER.Alternative name: small basic intrinisticprotein 2-1

Specificity: Predicted reactivity: not reactive in.

Application Details

Application Notes: Recommended Dilution 1: 8000 (ELISA), 1: 1000 with standard ECL (WB).

Buffer: 0.1 % sodium azide is added as preservative. For antibody re-suspendinginformation check the tube lable. Antibodies will detect target protein in a few µg of a crude preparation loaded perwell. If purified preparations of vacuolar and plasma membranes are

Storage: store at -20°C, make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin tubes briefly prior to opening them to avoid any losses thatmight occur from material adhering to the cap or sides of the tubes.

Research Area: Plant/Algal Physiology

Restrictions: For Research Use only

Images

crude membrane fraction/lane from Arabidopsis thaliana stems (1,3) and crudemembrane fraction from yeast cells expressing SIP2,1 (2,4) were separated on 12 %SDS-PAGE and blotted 1h to PVDF membrane (40 min. at 10 V using BioRadsemidry transfer). Filters were blocked 1h with 5 % low-fat milk powder in TBS-T(0.05% Triton X.100). Membranes were washed 5 times with TBS-T, each time in afresh polystyrene box and probed with anti-AtSIP2,1 antibodies (ABIN349680, 1:1000,1h) and secondary anti-rabbit (1:2000, 1 h). All steps were performed in RT withagitation. Peptide used to elicit anti-SIP2,1 antibodies have been incubated with antibodies -right panel.

anti-Probable Aquaporin SIP2-1 antibody (Image 2)

Publications

Publications: Ishikawa, Suga, Uemura et al.: "Novel type aquaporin SIPs are mainly localized to the ER membrane and show cell-specific expression in Arabidopsis thaliana." in: FEBS letters, Vol. 579, Issue 25, pp. 5814-20, 2005 (PubMed).