ATPase, H+ Transporting, Lysosomal V1 Subunit A (ATP6V1A) antibody

Antigen: ATPase, H+ Transporting, Lysosomal V1 Subunit A (ATP6V1A)

Clonality: Polyclonal

Host: Rabbit

Reactivity: Acetabularia, Barley (Hordeum vulgare), Rice (Oryza sativa), Pinus sylvestris, Pyrus, Ricinus communis

Application: ELISA, Western Blotting (WB)

Catalog no.: ABIN349685

Additional Information

Alternative name: Vacuolar H+-ATPase subunit A (V-ATPase, A)

Immunogen: purified native V-ATPase subunit A from Ricinus communis

Format: Serum

Description: V-ATPase subunit A is a catalytic subunit of V1 complex of vacuolar ATPase.This enzyme (EC=3.6.3.14) is involved in acidification process of variouscompartements of eucaryotic cell. This protein is coded by VHA-A gene.Alternative names: Vacuolar proton pump subunit alpha, vacuolar H(+)-ATPasesubunit A, V-ATPase 69 kDa subunit

Specificity: Predicted reactivity: dicots including: Arabidopsis thaliana, Gossypium mexicanum, Vitis vinifera,monocots including: Hordeum vulgare, trees: Populus trichocarpa.

Application Details

Application Notes: Recommended Dilution 1: 8000 (ELISA), 1: 2000 with standard ECL (WB).

Buffer: 0.1 % sodium azide is added as preservative. For antibody re-suspendinginformation check the tube lable. Antibodies will detect target protein in a few µg of a crude preparation loaded perwell. If purified preparations of vacuolar and plasma membranes are

Storage: store at -20°C, make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin tubes briefly prior to opening them to avoid any losses thatmight occur from material adhering to the cap or sides of the tubes.

Research Area: Plant/Algal Physiology

Restrictions: For Research Use only

Images

65ug/lane of purified vacuolar membranes from Vigna radiata L. (1,3) and purifiedV-ATPase, 7.4ug/lane (2,4) were separated on 12 % SDS-PAGE and blotted 1h toPVDF membrane (40 min. at 10 V using BioRad semidry transfer). Filters wereblocked 1h with 5 % low-fat milk powder in TBS-T (0.05% Triton X.100). Membraneswere washed 5 times with TBS-T, each time in a fresh polystyrene box and probedwith anti-V-ATPase subunit A antibodies (ABIN349685, 1:2000, 1h) and secondaryanti-rabbit (1:2000, 1 h). All steps were performed in RT with agitation. CBB - staining with Coomasie blue - left panel. Immunoblot - western blot detectoin -right panel.

Publications

Publications: Smart, Vojdani, Maeshima et al.: "Genes involved in osmoregulation during turgor-driven cell expansion of developing cotton fibers are differentially regulated." in: Plant physiology, Vol. 116, Issue 4, pp. 1539-49, 1998 (PubMed).

Kawamura, Arakawa, Maeshima et al.: "Tissue specificity of E subunit isoforms of plant vacuolar H(+)-ATPase and existence of isotype enzymes." in: The Journal of biological chemistry, Vol. 275, Issue 9, pp. 6515-22, 2000 (PubMed).

Matsuura-Endo, Maeshima, Yoshida: "Mechanism of the Decline in Vacuolar H -ATPase Activity in Mung Bean Hypocotyls during Chilling." in: Plant physiology, Vol. 100, Issue 2, pp. 718-722, 2006 (PubMed).