Vacuolar H+-ATPase Subunit B antibody

Antigen: Vacuolar H+-ATPase Subunit B

Clonality: Polyclonal

Host: Rabbit

Reactivity: Acetabularia, Barley (Hordeum vulgare), Rice (Oryza sativa), Pinus sylvestris, Pyrus, Vigna radiata

Application: ELISA, Western Blotting (WB)

Catalog no.: ABIN349686

Additional Information

Alternative name: Vacuolar H+-ATPase subunit B (V-ATPase, B)

Immunogen: purified native V-ATPase, subunit B from Vigna radiata

Description: V-ATPase, subunit B is a non-catalytic subunit of the peripheral V1 complex ofvacuolar ATPase. Alternative names: Vacuolar proton pump subunit B1, vacuolarH+-ATPase subunit B, V-ATPase 57 kDa subunit.

Specificity: Predicted reactivity: dicots including: Arabidopsis thaliana, Gossypium mexicanum, monocotsincluding: Hordeum vulgare, Oryza sativa, trees: Populus trichocarpa, moss:Physcomitrella patens, algae: Chlamydomonas reinhardtii,microalgae:Ostreococcus tauri.

Application Details

Application Notes: Recommended Dilution 1: 8000 (ELISA), 1: 2000 with standard ECL (WB).

Purity: ammonium sulfate purified

Buffer: 0.1 % sodium azide is added as preservative. For antibody re-suspendinginformation check the tube lable. Antibodies will detect target protein in a few µg of a crude preparation loaded perwell. If purified preparations of vacuolar and plasma membranes are

Storage: store at -20°C, make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin tubes briefly prior to opening them to avoid any losses thatmight occur from material adhering to the cap or sides of the tubes.

Research Area: Plant/Algal Physiology

Restrictions: For Research Use only

Images

65ug/lane of purified vacuolar membranes from Vigna radiata L. (1,3) and purifiedV-ATPase, 7.4ug/lane (2,4) were separated on 12 % SDS-PAGE and blotted 1h toPVDF membrane (40 min. at 10 V using BioRad semidry transfer). Filters were blocked1h with 5 % low-fat milk powder in TBS-T (0.05% Triton X.100). Membranes werewashed 5 times with TBS-T, each time in a fresh polystyrene box and probed withanti-V-ATPase subunit B antibodies (ABIN349686, 1:2000, 1h) and secondary anti-rabbit(1:2000, 1 h). All steps were performed in RT with agitation. CBB - staining with Coomasie blue - left panel. Immunoblot - western blot detectoin -right panel.

Publications

Publications: Smart, Vojdani, Maeshima et al.: "Genes involved in osmoregulation during turgor-driven cell expansion of developing cotton fibers are differentially regulated." in: Plant physiology, Vol. 116, Issue 4, pp. 1539-49, 1998 (PubMed).

Kawamura, Arakawa, Maeshima et al.: "Tissue specificity of E subunit isoforms of plant vacuolar H(+)-ATPase and existence of isotype enzymes." in: The Journal of biological chemistry, Vol. 275, Issue 9, pp. 6515-22, 2000 (PubMed).

Matsuura-Endo, Maeshima, Yoshida: "Mechanism of the Decline in Vacuolar H -ATPase Activity in Mung Bean Hypocotyls during Chilling." in: Plant physiology, Vol. 100, Issue 2, pp. 718-722, 2006 (PubMed).