Aquaporin, Plasma Membrane Intrinistic Protein 1-3 (PIP1,3) antibody

Antigen: Aquaporin, Plasma Membrane Intrinistic Protein 1-3 (PIP1,3)

Clonality: Polyclonal

Host: Rabbit

Reactivity: Rice (Oryza sativa)

Application: ELISA, Western Blotting (WB)

Catalog no.: ABIN349687

Additional Information

Immunogen: peptide derived from Oryza sativa PIP1-3 Q9SXF8

Format: Serum

Description: PIP1,3 is a plasma membrane aquaporine which facilitates trasport of wateracross cell membrane. Alternative names: aquaporin PIP 1-3, water channelprotein RWC3, RWC-3

Specificity: Predicted reactivity: monocots including: Hordeum vulgare, Stipa sp. Triticum aestivum.

Application Details

Application Notes: Recommended Dilution 1: 8000 (ELISA), 1: 2000 with standard ECL (WB).

Buffer: 0.1 % sodium azide is added as preservative. For antibody re-suspendinginformation check the tube lable. Antibodies will detect target protein in a few µg of a crude preparation loaded perwell. If purified preparations of vacuolar and plasma membranes are

Storage: store at -20°C, make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin tubes briefly prior to opening them to avoid any losses thatmight occur from material adhering to the cap or sides of the tubes.

Research Area: Plant/Algal Physiology

Restrictions: For Research Use only

Images

1.5ug of crude membrane fraction/lane from Oryza sativa L.Akitakomachi were separated on 12 % SDS-PAGE and blotted 1h toPVDF membrane (40 min. at 10 V using BioRad semidry transfer).Filters were blocked 1h with 5 % low-fat milk powder in TBS-T (0.05%Triton X.100). Membranes were washed 5 times with TBS-T, each timein a fresh polystyrene box and probed with anti-PIPs1,3 antibodies(ABIN349687, 1:1000, 1h) and secondary anti-rabbit (1:2000, 1 h). Allsteps were performed in RT with agitation. Below: Immunospecificity of anti.PIP1,3 antibodies to recombinant PIPproteins expressed in yeast cells. 1.5ug protein/lane. Most aquaporins show the dimerc forms in addition to monomer evenin the presence of SDS.

anti-Aquaporin, Plasma Membrane Intrinistic Protein 1-3 (PIP1,3) antibody (Image 2)

Publications

Publications: Sakurai, Ahamed, Murai et al.: "Tissue and cell-specific localization of rice aquaporins and their water transport activities." in: Plant & cell physiology, Vol. 49, Issue 1, pp. 30-9, 2008 (PubMed).