Aquaporin (PIP1s) antibody

Antigen: Aquaporin (PIP1s)

Synonyms: MIP, Aqp1, ACYPI006387, PIP2;1, PIP2-1, aquaporin, TIP1;1

Clonality: Polyclonal

Host: Rabbit

Reactivity: Rice (Oryza sativa)

Application: ELISA, Western Blotting (WB)

Catalog no.: ABIN349688

Additional Information

Alternative name: Aquaporin (PIP1,3)

Immunogen: KLH-conjugated peptide derived from Oryza sativa PIP1s

Format: Serum

Description: PIP1s - is a plasma membrane aquaporine which facilitates trasport of wateracross cell membrane.

Specificity: Predicted reactivity: not determined.

Application Details

Application Notes: Recommended Dilution 1: 8000 (ELISA), 1: 2000 with standard ECL (WB).

Buffer: 0.1 % sodium azide is added as preservative. For antibody re-suspendinginformation check the tube lable. Antibodies will detect target protein in a few µg of a crude preparation loaded perwell. If purified preparations of vacuolar and plasma membranes are

Storage: store at -20°C, make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin tubes briefly prior to opening them to avoid any losses thatmight occur from material adhering to the cap or sides of the tubes.

Research Area: Plant/Algal Physiology

Restrictions: For Research Use only

Images

1.5ug of crude membrane fraction/lane from Oryza sativa L. cv. Akitakomachi wereseparated on 12 % SDS-PAGE and blotted 1h to PVDF membrane (40 min. at 10 V usingBioRad semidry transfer). Filters were blocked 1h with 5 % low-fat milk powder in TBS-T(0.05% Triton X.100). Membranes were washed 5 times with TBS-T, each time in a freshpolystyrene box and probed with anti-PIP1s antibodies (ABIN349688, 1:2000, 1h) and secondaryanti-rabbit (1:2000, 1 h). All steps were performed in RT with agitation. Peptide used to elicti this antibody has been added to primary antibody incubation (right panel,+ +) and therefore specific signal has been depleted in this neutralization/immunocompetitionassay.

Publications

Publications: Sakurai, Ahamed, Murai et al.: "Tissue and cell-specific localization of rice aquaporins and their water transport activities." in: Plant & cell physiology, Vol. 49, Issue 1, pp. 30-9, 2008 (PubMed).