Aquaporin, Plasma Membrane Intrinistic Protein 2-5 (PIP2,5) antibody

Antigen: Aquaporin, Plasma Membrane Intrinistic Protein 2-5 (PIP2,5)

Clonality: Polyclonal

Host: Rabbit

Reactivity: Rice (Oryza sativa)

Application: ELISA, Western Blotting (WB)

Catalog no.: ABIN349691

Additional Information

Immunogen: KLH-conjugated peptide derived from Oryza sativa PIP2-5 Q8GRI8

Format: Serum

Description: PIP2,5 protein is an aquaporins which facilitates the transport of water and smallneutral molecules across cell membrane.

Specificity: Predicted reactivity: monocots including: Sorghum bicolor, Zea mays.

Application Details

Application Notes: Recommended Dilution 1: 8000 (ELISA), 1: 2000 with standard ECL (WB).

Buffer: 0.1 % sodium azide is added as preservative. For antibody re-suspendinginformation check the tube lable. Antibodies will detect target protein in a few µg of a crude preparation loaded perwell. If purified preparations of vacuolar and plasma membranes are

Storage: store at -20°C, make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin tubes briefly prior to opening them to avoid any losses thatmight occur from material adhering to the cap or sides of the tubes.

Research Area: Plant/Algal Physiology

Restrictions: For Research Use only

Images

5ug of crude membrane fraction/lane from Oryza sativa L. cv. Akitakomachi leaf bladeand root were separated on 12 % SDS-PAGE and blotted 1h to PVDF membrane (40 min.at 10 V using BioRad semidry transfer). Filters were blocked 1h with 5 % low-fat milkpowder in TBS-T (0.05% Triton X.100). Membranes were washed 5 times with TBS-T,each time in a fresh polystyrene box and probed with anti-PIP2,5 antibodies (ABIN349691,1:1000, 1h) and secondary anti-rabbit (1:2000, 1 h). All steps were performed in RT withagitation. Peptide used to elicti this antibody has been added to primary antibody incubation (rightpanel, + +) and therefore specific signal has been depleted in thisneutralization/immunocompetition assay.

Publications

Publications: Sakurai, Ahamed, Murai et al.: "Tissue and cell-specific localization of rice aquaporins and their water transport activities." in: Plant & cell physiology, Vol. 49, Issue 1, pp. 30-9, 2008 (PubMed).