Tonoplast Intrinistic Protein 2-2 (TIP2,2) (C-Term) antibody

Antigen: Tonoplast Intrinistic Protein 2-2 (TIP2,2)

Epitope: C-Term

Clonality: Polyclonal

Host: Rabbit

Reactivity: Rice (Oryza sativa)

Application: ELISA, Western Blotting (WB)

Catalog no.: ABIN349693

Additional Information

Alternative name: Tonoplast intrinistic protein 2-2 (C-terminal) (TIP2,2)

Immunogen: KLH-conjugated peptide derived from C-terminus of Oryza sativa TIP2-2 Q5Z6F0

Format: Serum

Description: TIP2-2 belongs to aquaporin protein family and facilitates transport of water andsmall neutral solutes across tonoplast membrane.

Specificity: Predicted reactivity: Oryza sativa.

Application Details

Application Notes: Recommended Dilution 1: 8000 (ELISA), 1: 2000 with standard ECL (WB).

Buffer: 0.1 % sodium azide is added as preservative. For antibody re-suspendinginformation check the tube lable. Antibodies will detect target protein in a few µg of a crude preparation loaded perwell. If purified preparations of vacuolar and plasma membranes are

Storage: store at -20°C, make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin tubes briefly prior to opening them to avoid any losses thatmight occur from material adhering to the cap or sides of the tubes.

Research Area: Plant/Algal Physiology

Restrictions: For Research Use only

Images

1.5ug of crude membrane fraction/lane from Oryza sativa L. cv. Akitakomachi leaf bladeand root were separated on 12 % SDS-PAGE and blotted 1h to PVDF membrane (40 min. at10 V using BioRad semidry transfer). Filters were blocked 1h with 5 % low-fat milk powderin TBS-T (0.05% Triton X.100). Membranes were washed 5 times with TBS-T, each time in afresh polystyrene box and probed with anti-TIP2,2 C-terminal antibodies (ABIN349693, 1:1000,1h) and secondary anti-rabbit (1:2000, 1 h). All steps were performed in RT with agitation. Peptide used to elicti this antibody has been added to primary antibody incubation (rightpanel, + +) and therefore specific signal has been depleted in thisneutralization/immunocompetition assay.

Publications

Publications: Sakurai, Ahamed, Murai et al.: "Tissue and cell-specific localization of rice aquaporins and their water transport activities." in: Plant & cell physiology, Vol. 49, Issue 1, pp. 30-9, 2008 (PubMed).