Epithelial Cell Adhesion Molecule (EPCAM) antibody

Antigen: Epithelial Cell Adhesion Molecule (EPCAM)

Synonyms: EGP, ESA, KSA, M4S1, MK-1, EGP-2, EGP34, EGP40, KS1/4, MIC18, TROP1, CO-17A, Ep-CAM, hEGP-2, CO17-1A, GA733-2, TACST-1, TACSTD1, sb:cb6, tacstd, zgc:77119, wu:fj17g02, EPCAM, tacstd1, MGC80540, MGC127627, egp, ksa, m4s1, mk-1, cd326, egp40, mic18, trop1, ep-cam, hegp-2, co17-1a, ga733-2

Clonality: Monoclonal (323-A3)

Host: Mouse

Reactivity: Human

Application: Flow Cytometry (FACS), Immunoprecipitation (IP), Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC)

Catalog no.: ABIN349703

Additional Information

Alternative name: CD326 / EpCAM

Immunogen: Human breast cancer MCF-7 cells

Format: Purified

Isotype: IgG1  (Matching secondary antibodies)

Clone: 323-A3

Description: CD326 / EpCAM (also known as ESA, EGP40, EGP-2, KSA1/4, CO17-1A, GA733-2,MOC31, Ber-EP4) is a 40 kDa transmembrane glycoprotein serving as adhesion molecule inthe basolateral membranes in a variety of epithelial cells. CD326 mediatescalcium-independent homotypic cell-cell adhesions. CD326 over-expression has beendetected in many epithelial tumours and is often associated with bad prognosis. It has beenused for diagnostics of (pre-) malignancies at early stages.

Specificity: The antibody 323/A3 recognizes CD326 / EpCAM, a marker of epithelial lineages, that isover-expressed in many carcinomas. This antibody does not react with: Rat.

Synonyms: EGP, ESA, KSA, M4S1, MK-1, EGP-2, EGP34, EGP40, KS1/4, MIC18, TROP1, CO-17A, Ep-CAM, hEGP-2, CO17-1A, GA733-2, TACST-1, TACSTD1, sb:cb6, tacstd, zgc:77119, wu:fj17g02, EPCAM, tacstd1, MGC80540, MGC127627, egp, ksa, m4s1, mk-1, cd326, egp40, mic18, trop1, ep-cam, hegp-2, co17-1a, ga733-2

Application Details

Application Notes: Immunoprecipitation - Recommended dilution: 1-4 µg / ml per 100-500 µg of protein in 1 ml lysate. - Recommended dilution: 1-4 µg/ml. Positive control: A-431, MCF-7, SW480 cell lines. Immunohistochemistry (paraffin sections) - Recommended dilution: 1-10 µg/ml Pretreatment: Tissue section digestion with pepsin (15 min at RT or 10 min at 37 o C, 1 mg / ml Tris-HCl, pH 2. 0). Positive tissue: Breast carcinoma. Immunocytochemistry - Recommended dilution: 1-10 µg/ml

Concentration: 1 mg/ml

Purity: > 95% (by SDS-PAGE)

Purification: Purified from cell culture supernatant by protein-A affinity chromatography.

Buffer: Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4

Storage: Store at 2-8 o C. Do not use after expiration date stamped on vial label. For long-term storage aliquot and store at -20 o C. Avoid freeze/thaw cycles.

Research Area: Cancer

Restrictions: For Research Use only

Publications

Publications: Edwards, Grzyb, Dressler et al.: "Monoclonal antibody identification and characterization of a Mr 43,000 membrane glycoprotein associated with human breast cancer." in: Cancer research, Vol. 46, Issue 3, pp. 1306-17, 1986 (PubMed).

Litvinov, Bakker, Gourevitch et al.: "Evidence for a role of the epithelial glycoprotein 40 (Ep-CAM) in epithelial cell-cell adhesion." in: Cell adhesion and communication, Vol. 2, Issue 5, pp. 417-28, 1995 (PubMed).

Litvinov, Velders, Bakker et al.: "Ep-CAM: a human epithelial antigen is a homophilic cell-cell adhesion molecule." in: The Journal of cell biology, Vol. 125, Issue 2, pp. 437-46, 1994 (PubMed).

Balzar, Bakker, Briaire-de-Bruijn et al.: "Cytoplasmic tail regulates the intercellular adhesion function of the epithelial cell adhesion molecule." in: Molecular and cellular biology, Vol. 18, Issue 8, pp. 4833-43, 1998 (PubMed).

Balzar, Briaire-de Bruijn, Rees-Bakker et al.: "Epidermal growth factor-like repeats mediate lateral and reciprocal interactions of Ep-CAM molecules in homophilic adhesions." in: Molecular and cellular biology, Vol. 21, Issue 7, pp. 2570-80, 2001 (PubMed).

Winter, Nagtegaal, van Krieken et al.: "The epithelial cell adhesion molecule (Ep-CAM) as a morphoregulatory molecule is a tool in surgical pathology." in: The American journal of pathology, Vol. 163, Issue 6, pp. 2139-48, 2003 (PubMed).

Kirman, Jenkins, Fowler et al.: "Naturally occurring antibodies to epithelial cell adhesion molecule (EpCAM)." in: Digestive diseases and sciences, Vol. 48, Issue 12, pp. 2306-9, 2004 (PubMed).

Bellone, Siegel, Cocco et al.: "Overexpression of epithelial cell adhesion molecule in primary, metastatic, and recurrent/chemotherapy-resistant epithelial ovarian cancer: implications for epithelial cell adhesion molecule-specific immunotherapy." in: International journal of gynecological cancer : official journal of the International Gynecological Cancer Society, Vol. 19, Issue 5, pp. 860-6, 2009 (PubMed).