Add to Basket
Order hotline:
+1 404 474 4654
+1 888 205 9894 (TF)

CD8a antibody (FITC)

Antigen

CD8a
Clonality Monoclonal (53-6-7)
Host
Reactivity
Alternatives

Mouse (Murine)
Conjugate
Application
Alternatives Flow Cytometry (FACS)
Catalog no. ABIN349708
Quantity 0.1 mg  (0.5 mg/ml)  (Variants)
Price 221.00 $   Plus shipping costs $35.00
Shipping to
Availability Ships within 3 to 5 Business Days

Additional Information
Application Details Alternatives Variants back to top

Immunogen Mouse spleen cells
Isotype IgG2a
Clone 53-6-7
Description The CD8a (CD8 alpha) subunit of CD8 T cell coreceptor is expressed in CD8 alpha/betaheterodimers on majority of MHC I-restricted conventional T cells and thymocytes and inCD8 alpha/alpha homodimers on subsets of memory T cells, intraepithelial lymphocytes, NKcells and dendritic cells. Regulation of CD8 beta level on T cell surface seems to be animportant mechanism to control their effector function. Assembly of CD8 alpha/beta but notalpha/alpha dimers is connected with formation or localization to the lipid rafts. Recruitingtriggered TCR complexes to these membrane microdomains as well as affinity of TCR toMHC I is modulated by CD8, thereby affecting the functional diversity of the TCR signaling.
Specificity The rat monoclonal antibody 53-6.7 recognizes mouse CD8a (32-34 kDa, alpha chain of theCD8 antigen).

Application Details
Alternatives Variants back to top

Application Notes The reagent is designed for Flow Cytometry analysis. Suggested working concentration is 2 µg/ml. Indicated dilution is recommended startingpoint for use of this product. Working concentrations should be determined by the investigator.
Concentration 0.5 mg/ml
Buffer The reagent is provided in phosphate buffered saline (PBS) containing 15 mM sodium azideand 0.2% (w/v) high-grade protease free Bovine Serum Albumin (BSA) as a stabilizing agent.
Storage Store in the dark at 2-8 o C. Do not freeze. Avoid prolonged exposure to light. Do not use after expiration date stamped on vial label.Short-term exposure to room temperature should not affect the quality of the reagent.However, if reagent is stored under any conditions other than those specified, the conditions must be verified by the user.
Research Area CD Antigens, Surface Receptors of Immune Cells, Major Histocompatibility Complex (MHC)
Restrictions For Research Use only

Alternatives
Application Details Variants back to top

Alternatives for antigen "CD8a", type "Antibodies"
Hosts Mouse (164), Rat (130), Rabbit (7), Chicken (1)
Reactivities Mouse (Murine) (149), Human (55), Chicken (34), Rat (Rattus) (31), Bird (Avian) (12), Pig (Porcine) (11), Dog (Canine) (4), Cat (Feline) (3), Sheep (Ovine) (3), Duck (1), Guinea Pig (1), Rabbit (1)
Applications Flow Cytometry (FACS) (270), Immunohistochemistry (Frozen Sections) (IHC (fro)) (45), Immunoprecipitation (IP) (35), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (22), Cytotoxicity Test (CyTox) (15), Immunohistochemistry (IHC) (10), Western Blotting (WB) (7), ELISA (5), Immunocytochemistry (ICC) (5), Functional Studies (Func) (4), Immunofluorescence (IF) (3), Blocking Antibody (Inhibition) (1), Blocking Antibody (Inhibition) (1), Immunohistochemistry (Paraformaldehyde) (IHC (pfa)) (1)
Conjugates FITC (60), PE (43), Biotin (39), APC (19), Cy5 (10), RPE (10), Cy5,PE (7), Cy5.5 (5), Cy7,PE (4), SPRD (4), PE,Cy7 (2), APC,Cy7 (1), PE,Cy5 (1), PE,Cy5.5 (1), PE,DY590 (1), PE,DY647 (1), PerCP (1), Texas Red (TR) (1)