Mucin 1, Cell Surface Associated (MUC1) antibody

Antigen: Mucin 1, Cell Surface Associated (MUC1)

Synonyms: EMA, PEM, PUM, KL-6, MAM6, PEMT, CD227, H23AG, MGC188069, MUC1

Clonality: Monoclonal (E29)

Host: Mouse

Application: Immunohistochemistry (Frozen Sections) (IHC (fro))

Catalog no.: ABIN349856

Additional Information

Alternative name: Human Epithelial Membrane Antigen (EMA) 265-400 kDa

Immunogen: Delipidated extract of human cream

Format: Supernatant, Liquid

Isotype: IgG2a  (Matching secondary antibodies)

Clone: E29

Specificity: Recognizes epithelial membrane antigen (EMA), a heavily glycosylated protein of 265-400 kDa. This antibody stains normal and neoplastic epithelium including adenocarcinomas from a range of sites, squamous and transitional cell carcinomas and mesotheliomas. Strong staining of glandular epithelium is seen, but staining of squamous epithelium may be rather patchy. Positive staining with this antibody demonstrates epithelial origin of tumors.

Application Details

Application Notes: Suitable for use in Immunohistochemistry (1:10 ? 1:40 with frozen or paraffin sections). Paraffin sections do not require protein digestion pretreatment or antigen retrieval using heat treatment prior to staining. Each laboratory should determine an optimum working titer for use in its particular application. Other applications have not been tested but use in such assays should not necessarily be excluded.

Concentration: Not determined

Purification: Not applicable. Source: Tissue Culture

Buffer: PBS containing 1% BSA. Preservative: 0.09% Sodium azide

Storage: Short term (up to 2 weeks) store at 2-8°C. Long term store at -20°C. Avoid multiple freeze/thaw cycles.

Restrictions: For Research Use only