MAS-Related GPR, Member X1/3/4 (MRGPRX1/3/4) antibody

Antigen: MAS-Related GPR, Member X1/3/4 (MRGPRX1/3/4)

Synonyms: GPCR, MRGX1, SNSR4, Snsr1, Mrgprc11

Clonality: Polyclonal

Host: Rabbit

Application: Immunohistochemistry (IHC), Western Blotting (WB)

Catalog no.: ABIN349968

Additional Information

Alternative name: Mas-related G-protein coupled receptor member X1, X3 and X4 (MRGPRX1, 3, 4)

Swiss-Prot: Q96LB2

Immunogen: A synthetic peptide from human and monkey (macaca mulatta, macaca ascicularis) Mas-related G-protein coupled receptor member X1, X3 and X4 (MRGPRX1, MRGPRX3, MRGPRX4) conjugated to an immunogenic carrier protein was used as the immunogen.

Format: Lyophilized

Description: FUNCTION: Orphan receptor. Probably involved in the function of nociceptive neurons. May regulate nociceptor function and/or development, including the sensation or modulation of pain. Potently activated by enkephalins including BAM22 (bovine adrenal medulla peptide 22) and BAM (8-22). BAM22 is the most potent compound and evoked a large and dose-dependent release of intracellular calcium in stably transfected cells. G(alpha)q proteins are involved in the calcium-signaling pathway. SUBCELLULAR LOCATION: Cell membrane; Multi-pass membrane protein. TISSUE SPECIFICITY: Uniquely localized in a subset of small dorsal root and trigeminal sensory neurons. Also known as: Mas-related G-protein coupled receptor member X1, X3, X4; Sensory neuron-specific G-protein coupled receptor 3/4, MRGX1, SNSR3, SNSR4, GPCR, MAS-related GPR, member X1, MRGX3, SNSR1, SNSR2, Sensory neuron-specific G-protein coupled receptor 1/2, Sensory neuron-specific G-protein coupled receptor 5/6, MRGX4, SNSR5, SNSR6, MAS-related GPR, member X3; MRGPRX4, MRGPRX3, MRGPRX1.

Specificity: Appears to be specific for MRGPRX1, 3 and 4.

Application Details

Application Notes: IHC, WB. A dilution of 1 : 300 to 1 : 2000 is recommended. The optimal dilution should be determined by the end user. Not yet tested in other applications.

Purity: whole serum

Storage: Maintain the lyophilised/reconstituted antibodies frozen at -20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.

Restrictions: For Research Use only

Publications

Publications: Dong, Han, Zylka et al.: "A diverse family of GPCRs expressed in specific subsets of nociceptive sensory neurons." in: Cell, Vol. 106, Issue 5, pp. 619-32, 2001 (PubMed).

Lembo, Grazzini, Groblewski et al.: "Proenkephalin A gene products activate a new family of sensory neuron--specific GPCRs." in: Nature neuroscience, Vol. 5, Issue 3, pp. 201-9, 2002 (PubMed).

Takeda, Kadowaki, Haga et al.: "Identification of G protein-coupled receptor genes from the human genome sequence." in: FEBS letters, Vol. 520, Issue 1-3, pp. 97-101, 2002 (PubMed).

Kaisho, Watanabe, Nakata et al.: "Transgenic rats overexpressing the human MrgX3 gene show cataracts and an abnormal skin phenotype." in: Biochemical and biophysical research communications, Vol. 330, Issue 3, pp. 653-7, 2005 (PubMed).