G Protein-Coupled Receptor MRGX2 (MRGPRX2) (4th cytoplasmic loop) antibody

Antigen: G Protein-Coupled Receptor MRGX2 (MRGPRX2)

Synonyms: MRGX2

Epitope: 4th cytoplasmic loop

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Application: Immunohistochemistry (IHC), Western Blotting (WB)

Catalog no.: ABIN349969

Additional Information

Alternative name: Mas-related G-protein coupled receptor member X2 (MRGPRX2) (4th cytoplasmic loop)

Swiss-Prot: Q96LB1

Immunogen: A synthetic peptide from the 4th cytoplasmic loop of human & monkey Mas-related G-protein coupled receptor member X2 (MRGPRX2) conjugated to an immunogenic carrier protein was used as the immunogen.

Format: Lyophilized

Description: FUNCTION: Orphan receptor. Probably involved in the function of nociceptive neurons. May regulate nociceptor function and/or development, including the sensation or modulation of pain. Cortistatin-14 seems to be a high potency ligand at this receptor. Cortistatin has several biological functions including roles in sleep regulation locomotor activity, and cortical function. In receptor-expressing cells, cortistatin-stimulated increases in intracellular Ca(2+) but had no effect on basal or forskolin-stimulated cAMP levels, suggesting that this receptor is G(q)-coupled. SUBCELLULAR LOCATION: Cell membrane; Multi-pass membrane protein. TISSUE SPECIFICITY: Has a limited expression profile, both peripheral and within the central nervous system, with highest levels in dorsal root ganglion. Also known as: Mas-related G-protein coupled receptor member X2, MRGPRX2, MAS-related GPR, member X2.

Specificity: Appears to be specific for MRGPRX2.

Application Details

Application Notes: IHC, WB (confirmed by recombinant protein). A dilution of 1 : 200 to 1 : 2000 is recommended. The optimal dilution should be determined by the end user.

Purity: whole serum

Storage: Maintain the lyophilised/reconstituted antibodies frozen at -20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.

Restrictions: For Research Use only

Publications

Publications: Dong, Han, Zylka et al.: "A diverse family of GPCRs expressed in specific subsets of nociceptive sensory neurons." in: Cell, Vol. 106, Issue 5, pp. 619-32, 2001 (PubMed).

Takeda, Kadowaki, Haga et al.: "Identification of G protein-coupled receptor genes from the human genome sequence." in: FEBS letters, Vol. 520, Issue 1-3, pp. 97-101, 2002 (PubMed).

Robas, Mead, Fidock: "MrgX2 is a high potency cortistatin receptor expressed in dorsal root ganglion." in: The Journal of biological chemistry, Vol. 278, Issue 45, pp. 44400-4, 2003 (PubMed).

Yang, Liu, Lin et al.: "Adaptive evolution of MRGX2, a human sensory neuron specific gene involved in nociception." in: Gene, Vol. 352, pp. 30-5, 2005 (PubMed).