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ATG5 Autophagy Related 5 Homolog (S. Cerevisiae) (ATG5) (AA 100-150) antibody

Details for Product No. ABIN349971
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Antigen
Synonyms
ATG5, CG1643, DmAtg5, Dmel\\CG1643, atg5, apg5l, zgc:100934, ATATG5, AUTOPHAGY 5, MKP11.20, MKP11_20, APG5, APG5-LIKE, APG5L, ASP, hAPG5, 2010107M05Rik, 3110067M24Rik, AW319544, Apg5l, Atg5l, C88337,  ... show more
ATG5, CG1643, DmAtg5, Dmel\\CG1643, atg5, apg5l, zgc:100934, ATATG5, AUTOPHAGY 5, MKP11.20, MKP11_20, APG5, APG5-LIKE, APG5L, ASP, hAPG5, 2010107M05Rik, 3110067M24Rik, AW319544, Apg5l, Atg5l, C88337, Paddy show less
Epitope
AA 100-150
(37), (16), (10), (9), (4), (4), (4), (4), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1)
Reactivity
Human, Mouse (Murine)
(145), (68), (54), (23), (20), (13), (12), (7), (7), (6), (2), (1), (1), (1), (1)
Host
Rabbit
(105), (31), (12), (3), (2)
Clonality
Polyclonal
Conjugate
Un-conjugated
(5), (5), (5), (4), (4), (4), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application
Immunohistochemistry (IHC), Western Blotting (WB)
(132), (76), (70), (23), (17), (10), (7), (4), (3), (3), (1)
Pubmed 5 references available
Quantity 150 µL
Options
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Catalog No. ABIN349971
454.67 $
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Immunogen A synthetic peptide from aa region 100-150 of human ATG5 conjugated to an immunogenic carrier protein has been used as the antigen. The peptide is homologous in mouse, xenopus and chicken.
Specificity Specific for ATG5.
Purification Whole serum
Alternative Name ATG5
Background ATG5 is an important element forautophagy and may also play an important role in the apoptotic process. Following conjugation to ATG12, the conjugate participates in the formation of autophagosome. While the mRNA is ubiquitously transcribed its expression is highly upregulated in apoptotic cells. Also known as: APG5-like, ATG5, ASP, APG5, APG5L, hAPG5, apoptosis-specific protein.
Application Notes Use at a dilution of 1:300 to 1: 2000.
The optimal dilution should be determined by the end user.
Not yet tested in other applications.
Restrictions For Research Use only
Format Lyophilized
Reconstitution Reconstitute in 150 µL of sterile water. Centrifuge to remove any insoluble material.
Handling Advice Avoid freeze and thaw cycles.
Storage 4 °C/-20 °C
Storage Comment Maintain the lyophilised/reconstituted antibodies frozen at -20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.
Expiry Date 12 months
General Grand, Milner, Mustoe et al.: "A novel protein expressed in mammalian cells undergoing apoptosis." in: Experimental cell research, Vol. 218, Issue 2, pp. 439-51, 1995 (PubMed).

Hammond, Brunet, Johnson et al.: "Homology between a human apoptosis specific protein and the product of APG5, a gene involved in autophagy in yeast." in: FEBS letters, Vol. 425, Issue 3, pp. 391-5, 1998 (PubMed).

Mizushima, Sugita, Yoshimori et al.: "A new protein conjugation system in human. The counterpart of the yeast Apg12p conjugation system essential for autophagy." in: The Journal of biological chemistry, Vol. 273, Issue 51, pp. 33889-92, 1999 (PubMed).

Schmeiser, Armstrong, Hammond et al.: "Assignment of the yeast APG5 human homologue APG5L to chromosome band 6q21 by fluorescence in situ hybridisation." in: Cytogenetics and cell genetics, Vol. 87, Issue 3-4, pp. 213-4, 2000 (PubMed).

Simonsen, Birkeland, Gillooly et al.: "Alfy, a novel FYVE-domain-containing protein associated with protein granules and autophagic membranes." in: Journal of cell science, Vol. 117, Issue Pt 18, pp. 4239-51, 2004 (PubMed).

Validation Images
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