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ATG3 antibody (ATG3 Autophagy Related 3 Homolog (S. Cerevisiae))

Details for Product anti-ATG3 Antibody No. ABIN349972, Supplier: Log in to see
Antigen
  • 2610016C12Rik
  • APG3
  • APG3-LIKE
  • Apg3l
  • APG3L
  • ATATG3
  • atg3
  • Atg3l
  • im:6910145
  • K11J9.1
  • K11J9_1
  • PC3-96
  • PIG-1
  • Pig1
  • wu:fb15g12
  • zgc:64156
Reactivity
Human, Mouse (Murine), Rat (Rattus)
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53
51
4
3
1
Host
Sheep
130
47
3
Clonality
Polyclonal
Conjugate
This ATG3 antibody is un-conjugated
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11
6
5
5
4
3
3
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
Application
Immunohistochemistry (IHC), Western Blotting (WB)
108
86
41
24
18
18
18
10
4
1
1
Supplier
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Immunogen A synthetic peptide from human ATG3 conjugated to an immunogenic carrier protein has been used as the antigen. The antigen is homologous in rat and mouse.
Isotype IgG
Specificity Specific for ATG3.
Alternative Name ATG3 (ATG3 Antibody Abstract)
Background Autophagy is a process of bulk degradation of cytoplasmic components by the lysosome or vacuole. Human ATG3 displays the same enzymatic characteristics in vitro as yeast Apg3, a protein-conjugating enzyme essential forautophagy.
Function: GABARAPL1 (GABARAPL2 or GABARAP or MAP1LC3)-modifier protein conjugating enzyme involved in its E2-like covalent binding to PE. ATG7 (E1-like enzyme) facilitates this reaction by forming an E1-E2 complex with ATG3 (E2-like enzyme). Preferred substrate is MAP1LC3A. Formation of the GABARAPL1-PE conjugate is essential forautophagy.
Subcellular location: Cytoplasm
Tissue specificity: Widely expressed, with a highest expression in heart, skeletal muscle, kidney, liver and placenta. Also known as: ATG3, APG3, APG3L, APG3-like, hApg3, protein PC3-96, FLJ22125, MGC15201, DKFZp564M1178.
Application Notes Use at a concentration of 10-50 µg/ml.
The optimal working concentration should be determined by the end user.
Not yet tested in other applications.
Restrictions For Research Use only
Format Lyophilized
Reconstitution Reconstitute in 500 µL of sterile water. Centrifuge to remove any insoluble material.
Handling Advice Avoid freeze and thaw cycles.
Storage 4 °C/-20 °C
Storage Comment Maintain the lyophilised/reconstituted antibodies frozen at -20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.
Expiry Date 12 months
Background publications Brill, Salomon, Ficarro, Mukherji, Stettler-Gill, Peters: "Robust phosphoproteomic profiling of tyrosine phosphorylation sites from human T cells using immobilized metal affinity chromatography and tandem mass spectrometry." in: Analytical chemistry, Vol. 76, Issue 10, pp. 2763-72, 2004 (PubMed).

Tanida, Tanida-Miyake, Komatsu, Ueno, Kominami: "Human Apg3p/Aut1p homologue is an authentic E2 enzyme for multiple substrates, GATE-16, GABARAP, and MAP-LC3, and facilitates the conjugation of hApg12p to hApg5p." in: The Journal of biological chemistry, Vol. 277, Issue 16, pp. 13739-44, 2002 (PubMed).